... aseptically add 27. 0mL of sterile lactose solution, 10.0mL of sterile Bromthymol Blue solution,2.0mL of sterile sodium thiosulfate solution, 1.2mL of sterile glucose so-lution, and 1.0mL of sterile ... 45°–50°C. Aseptically add 10.0mL of sterile horse serum, 1.0mL of sterile ampicillin solution, 0.25mL of ster-ile urea solution, 0.1mL of sterile nystatin solution, and 0.1mL of steriletripeptide solution. ... Preparation of Medium: To 950.0mL of cooled, sterile anaerobicagar base, aseptically add 10.0mg of vitamin K1 and 50.0mL of sterile, de-fibrinated sheep blood.Use: For the isolation of anaerobes.Brucella...