Handbook of Microbiological Media, Fourth Edition part 25 ppt

... digest of casein 5.0g Peptic digest of animal tissue 5.0g Potato, solids from 125g infusion 4.5g Rabbit blood, sterile defibrinated 150.0mL Glycerol 10.0mL pH 6.7± 0.2 at 25 C Preparation of Medium: ... medium. Preparation of Medium: Add a few drops of H 2 SO 4 to the distilled water to retard precipitation of the metal salts. Add components, except for Metals “44” and vitamin s...

Ngày tải lên: 03/07/2014, 18:20

... the numbers of particular species of microorganisms. Most often, mixtures of growth factors are used in microbiological media. Acid hydrolysates of casein commonly are used as sources of amino acids. ... Peptone A mixture of peptones composed of equal parts of pancreatic digest of casein and peptic digest of animal tissue. Proteose Peptone A specialized peptone prepared...

Ngày tải lên: 03/07/2014, 18:20

... 5.2g Preparation of A & A FeEDTA Solution: Dissolve 5.2g of KOH in 186.0mL of distilled/deionized water. Add 20.4g of disodium EDTA·2H 2 O. Add 13.7g of FeSO4·7H2O to 364.0mL of distilled/deion- ized ... 7.75mL: Acetic acid 4.25mL Propionic acid 1.50mL Butyric acid 1.0mL DL-2-Methyl butyric acid 0.25mL iso-Butyric acid 0.25mL iso-Valeric acid 0.25mL n-Valeric acid 0.25mL Prepa...

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... 0.05g Nicotinamide 0.05g Pyridoxal·HCl 0.05g Riboflavin 0.05g Pyridoxamine·HCl 0.025g Folic acid 0.025g Thiamine·HCl 0.15g Biotin 0.0125mg DL-Thioctic acid 0.5mL Preparation of Vitamin Solution: Add components ... at 35°C overnight. Transfer 0.5mL aliquots of nutrient broth with bacterial suspension to each of ten 1.0L Erlenmeyer flasks, each containing 250 .0mL of nutrient broth. In...

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... 15 min at 15 psi pressure–121°C. Preparation of Medium: Aseptically combine 500.0mL of sterile solution A, 500.0mL of sterile solution C, and 1.0mL of sterile solution B. Mix thoroughly. Pour ... Preparation of Medium: Aseptically combine 1.0L of sterile solu- tion A and 1.0mL of sterile solution B. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For...

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... 45°–50°C. Aseptically combine 25. 0mL of washed cells and 250 .0mL of cooled, sterile agar solution. Mix thor- oughly. Pour into sterile Petri dishes. Use: For the cultivation of freshwater Myxobacterium ... temperature for 20 min. Add 20.0mL of dis- tilled/deionized H 2 O. Filter through a layer of cheesecloth. To 90– 100.0mL of filtrate add 0.8% NaCl solution to bring volume...

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... per 25. 0mL: L-Cysteine·HCl·H 2 O 0.25g Na 2 S·9H 2 O 0.25g Preparation of Alkaline Solution: Add components to 25. 0mL of distilled/deionized water. Mix thoroughly. Prepare freshly. Preparation of ... phase of 98% CO 2 + 2% H 2 . Cool slightly. Alkaline Solution: Composition per 25. 0mL: L-Cysteine·HCl·H 2 O 0.25g Na 2 S·9H 2 O 0.25g Preparation of Alkaline Solution: Add compo...

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... 1035) Composition per liter: NaCl 5.6g Pancreatic digest of casein 1.8g KH 2 PO 4 1.0g Papaic digest of soybean meal 0.6g pH 6.5 ± 0.2 at 25 C Preparation of Medium: Add components to distilled/deionized water ... from other coliform colonies which have a salmon to red color. As part of an additional confirmation of E. coli, the inclusion of tryptophan improves the indole reac...

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... combine 870.0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... Medium: To 1.0L of sterile solution A, add in or- der: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of sterile solution D, 10.0mL of sterile solution E, 1....

Ngày tải lên: 03/07/2014, 18:20

... combine 870.0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... 5.0mg Riboflavin 5.0mg Thiamine·HCl 5.0mg Biotin 5.0mg Folic acid 5.0mg Vitamin B 12 5.0mg Hemin 5.0mg Preparation of Solution E (Vitamin Solution): Add 1,4-naphtho- quinone and hemi...

Ngày tải lên: 03/07/2014, 18:20