Handbook of Microbiological Media, Fourth Edition part 20 pptx

... 20 min. Add 20. 0mL of dis- tilled/deionized H 2 O. Filter through a layer of cheesecloth. To 90– 100.0mL of filtrate add 0.8% NaCl solution to bring volume to 125.0mL. Autoclave for 20 min at 15 ... 100.0mL: Hemin 1.0g NaOH (1N solution) 20. 0mL © 201 0 by Taylor and Francis Group, LLC BAGG Broth Base with Glycerol 191 Preparation of Hemin Solution: Add hemin to 20. 0mL of...

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... the numbers of particular species of microorganisms. Most often, mixtures of growth factors are used in microbiological media. Acid hydrolysates of casein commonly are used as sources of amino acids. ... 1924 Z 1953 INDEX 1957 © 201 0 by Taylor and Francis Group, LLC Handbook of MICROBIOLOGICAL MEDIA Fourth Edition Ronald M. Atlas Washington, D.C. CRC Press is an imprin...

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... 5.0mL of nutrient broth. Incubate at 35°C overnight. Transfer 0.5mL aliquots of nutrient broth with bacterial suspension to each of ten 1.0L Erlenmeyer flasks, each containing 250.0mL of nutrient ... com- bine 500.0mL of solution 1 with 500.0mL of solution 2. Nutrient Agar: Composition per liter: Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g Preparation of Nu...

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... Hanks’ salts (MEMH) and 1.0L of sterile L 15 medium, modified Leibovitz. Mix thoroughly. Immedi- ately prior to use, aseptically add 200 .0mL of fetal calf serum and 50.0mL of NaHCO 3 solution. Mix ... 45°–50°C. Preparation of Cellulose Overlay Agar: Aseptically combine 350.0mL of cooled, sterile solution A and 650.0mL of cooled, sterile solution B. Mix thoroughly. Preparation...

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... pressure–121°C. Preparation of Medium: To 1.0L of sterile solution A, add in or- der: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of sterile solution D, 10.0mL of sterile solution E, 1.0mL of sterile ... freshly. Preparation of Medium: To 920. 0mL of cooled, sterile solution A, aseptically and anaerobically add in the following order: 1.0mL of ster- il...

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... combine 870.0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... 5.0mg Pyridoxine·HCl 5.0mg Riboflavin 5.0mg Thioctic acid 5.0mg NaOH (0.1N solution) 5.0mL Preparation of Vitamin Solution: Add thioctic acid, 1,4-naphtho- quinone, and hemin to 5.0mL...

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... 1.0mL of sterile solution E, 1.0mL of sterile solution F, 10.0mL of sterile solution G, 1.0mL of sterile solution H, 6.6mL of sterile solution I, 20. 0mL of sterile solution J, and 10.0mL of sterile ... 10.0mL of sterile solution D, 1.0mL of sterile solution E, 10.0mL of sterile solution F, 6.6mL of sterile solution G, 20. 0mL of sterile solution H, and 10.0mL of s...

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... solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, 6.6mL of sterile solution F, 20. 0mL of sterile solution G, and 10.0mL of sterile solution ... pressure–121°C. Preparation of Medium: To 1.0L of sterile solution A, add in the fol- lowing order: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of steril...

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... freshly. Preparation of Medium: To 920. 0mL of cooled, sterile solution A, aseptically and anaerobically add in the following order: 1.0mL of ster- ile solution B, 50.0mL of sterile solution C, 10.0mL of sterile ... anaerobically add 10.0mL of sterile Na 2 S·9H 2 O solution and 5.0g of sterile powdered sulfur. Mix thor- oughly. Final pH of medium should be 5.5. Use: For the cu...

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... hydrolysate 20. 0g Soya lecithin 5.0g Polysorbate 20 40.0 ml pH 7.3 ± 0.2 at 25°C Source: This medium, without polysorbate 20, is available as a pre- mixed powder from HiMedia. Preparation of Medium: ... Cool to 25°C. Use: For the cultivation of anaerobic, microaerophilic, and aerobic microorganisms. For use in sterility testing of a variety of specimens. © 201 0 by Taylor and F...

Ngày tải lên: 03/07/2014, 18:20