Handbook of Microbiological Media, Fourth Edition part 15 docx

... pressure. Au- toclave for 15 min at 15 psi pressure–121°C. Prior to inoculation of cultures, add 0.25mL of sterile Na 2 S·9H 2 O solution to each tube con- taining 9.75mL of sterile basal medium. ... pressure. Autoclave for 15 min at 15 psi pressure–121°C. Prior to inoculation of cultures, add 0.25mL of sterile Na 2 S·9H 2 O solution to each tube containing 9.75mL of steri...

Ngày tải lên: 03/07/2014, 18:20

... 10.0mL of CaCl 2 solution, 10.0mL of MgSO 4 ·7H 2 O solution, 10.0mL of NaNO 3 solution, 10.0mL of K 2 HPO 4 solution, 10.0mL of KH 2 PO 4 solution, and 10.0mL of NaCl solution to distilled/deionized water ... tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the isolation and cultivation of algae. Algal Proteose Agar Composition per liter: Agar...

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... Adjust the volume of each tube to 10.0mL with distilled/deionized water. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the determination of the vitamin B 12 content of pharmaceuti- cal ... Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 10.0mL of sterile Wolfe’s mineral solution, 10.0mL of sterile Wolfe’s vitamin so- lution, and 10...

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... differentiation of E. coli O157. The addition of rhamnose aids in the differentiation of Escherichia coli O157 from background flora. Cefixime reduces the level of competing flora, particularly ... boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to th...

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... 25.0mL of 95% ethanol solution. Aseptically combine 20.0mL of sterile K 2 CO 3 solution and 15. 0mL of sterile etha- nol. Preparation of Medium: Add 35.0mL of sterile Part B to 965.0mL of sterile, ... for 15 min at 15 psi pressure–121°C. Aseptically add 0.2mL of sterile glucose solu- tion, 0.1mL of sterile L-Cysteine·HCl·H 2 O solution, 0.1mL of sterile Na 2 S·9H 2...

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... cultivation of a wide variety of microorganisms. For use as a base for the preparation of blood agar and for general laboratory proce- dures. Dextrose Agar Composition per liter: Agar 15. 0g Glucose ... solution 15. 0mL pH 7.4 ± 0.2 at 25°C Glucose Solution: Composition per 15. 0mL: Glucose 3.0g Preparation of Glucose Solution: Add glucose to distilled/deion- ized water and bring...

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... min. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 100.0mL of sterile glucose solution, 10.0mL of sterile Wolfe’s mineral solution, 10.0mL of sterile Wolfe’s vitamin ... the isolation and cultivation of Serratia marcescens. DNase Test Agar Composition per liter: Agar 15. 0g Pancreatic digest of casein 15. 0g NaCl 5.0g Papaic digest of soybe...

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... CO 2 . Filter sterilize. Preparation of Medium: Inject 1.0 ml of solution B and 0.2 ml of solution C in each tube of solution A. Use: For the cultivation of Rhodovibrio sodomensis=Rhodospirillum sodomense. DSM ... Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the maintenance of lactic acid bacteria and differentiatio...

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... cultivation and maintenance of Taylorella equigenitalis. Eugon Blood Agar (Eugonagar™) Composition per liter: Agar 15. 0g Pancreatic digest of casein 15. 0g Glucose 5.5g Papaic digest of soybean meal 5.0g NaCl ... pressure–121°C. Preparation of Medium: To each tube containing 8.9mL of sterile solution A, add (using a syringe) 1.0mL of sterile solution B, 0.1mL of sterile solut...

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... extract 2.5g Preparation of Yeast Extract Solution: Add components to 15. 0mL distilled/deionized water. Autoclave for 15 min at 15 psi pres- sure–121°C. Preparation of Medium: Add components, ... or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of fungi. Fungi Kimmig Agar Composition per liter: Glucose 19.0g Agar 15. 0g Peptone 15. 0g NaCl...

Ngày tải lên: 03/07/2014, 18:20