Handbook of Microbiological Media, Fourth Edition part 15 docx
Handbook of Microbiological Media, Fourth Edition part 15 docx
... pressure. Au-
toclave for 15 min at 15 psi pressure–121°C. Prior to inoculation of
cultures, add 0.25mL of sterile Na
2
S·9H
2
O solution to each tube con-
taining 9.75mL of sterile basal medium. ... pressure. Autoclave for 15 min at
15 psi pressure–121°C. Prior to inoculation of cultures, add 0.25mL of
sterile Na
2
S·9H
2
O solution to each tube containing 9.75mL of steri...
Handbook of Microbiological Media, Fourth Edition part 8 docx
... 10.0mL of CaCl
2
solution, 10.0mL of MgSO
4
·7H
2
O solution,
10.0mL of NaNO
3
solution, 10.0mL of K
2
HPO
4
solution, 10.0mL of
KH
2
PO
4
solution, and 10.0mL of NaCl solution to distilled/deionized
water ... tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C.
Use: For the isolation and cultivation of algae.
Algal Proteose Agar
Composition per liter:
Agar...
Handbook of Microbiological Media, Fourth Edition part 18 docx
... Adjust the volume of each tube
to 10.0mL with distilled/deionized water. Autoclave for 15 min at 15
psi pressure–121°C.
Use: For the determination of the vitamin B
12
content of pharmaceuti-
cal ... Autoclave for 15 min at
15 psi pressure–121°C. Aseptically and anaerobically add 10.0mL of
sterile Wolfe’s mineral solution, 10.0mL of sterile Wolfe’s vitamin so-
lution, and 10...
Handbook of Microbiological Media, Fourth Edition part 34 docx
... differentiation of E. coli O157. The addition of rhamnose
aids in the differentiation of Escherichia coli O157 from background
flora. Cefixime reduces the level of competing flora, particularly ... boiling. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 50.0mL
of sterile blood and 1.5mL of sterile water-lysed blood solution (one
part blood to th...
Handbook of Microbiological Media, Fourth Edition part 42 docx
... 25.0mL of 95% ethanol solution. Aseptically
combine 20.0mL of sterile K
2
CO
3
solution and 15. 0mL of sterile etha-
nol.
Preparation of Medium: Add 35.0mL of sterile Part B to 965.0mL
of sterile, ... for 15 min at
15 psi pressure–121°C. Aseptically add 0.2mL of sterile glucose solu-
tion, 0.1mL of sterile
L-Cysteine·HCl·H
2
O solution, 0.1mL of sterile
Na
2
S·9H
2...
Handbook of Microbiological Media, Fourth Edition part 60 docx
... cultivation of a wide variety of microorganisms. For use as a
base for the preparation of blood agar and for general laboratory proce-
dures.
Dextrose Agar
Composition per liter:
Agar 15. 0g
Glucose ... solution 15. 0mL
pH 7.4 ± 0.2 at 25°C
Glucose Solution:
Composition
per 15. 0mL:
Glucose 3.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring...
Handbook of Microbiological Media, Fourth Edition part 61 docx
... min. Autoclave for 15 min at 15 psi pressure–121°C.
Aseptically and anaerobically add 100.0mL of sterile glucose solution,
10.0mL of sterile Wolfe’s mineral solution, 10.0mL of sterile Wolfe’s
vitamin ... the isolation and cultivation of Serratia marcescens.
DNase Test Agar
Composition per liter:
Agar 15. 0g
Pancreatic digest of casein 15. 0g
NaCl 5.0g
Papaic digest of soybe...
Handbook of Microbiological Media, Fourth Edition part 62 docx
... CO
2
. Filter sterilize.
Preparation of Medium: Inject 1.0 ml of solution B and 0.2 ml of
solution C in each tube of solution A.
Use: For the cultivation of Rhodovibrio sodomensis=Rhodospirillum
sodomense.
DSM ... Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the maintenance of lactic acid bacteria and differentiatio...
Handbook of Microbiological Media, Fourth Edition part 67 docx
... cultivation and maintenance of Taylorella equigenitalis.
Eugon Blood Agar
(Eugonagar™)
Composition per liter:
Agar 15. 0g
Pancreatic digest of casein 15. 0g
Glucose 5.5g
Papaic digest of soybean meal 5.0g
NaCl ... pressure–121°C.
Preparation of Medium: To each tube containing 8.9mL of sterile
solution A, add (using a syringe) 1.0mL of sterile solution B, 0.1mL of
sterile solut...
Handbook of Microbiological Media, Fourth Edition part 72 docx
... extract 2.5g
Preparation of Yeast Extract Solution: Add components to
15. 0mL distilled/deionized water. Autoclave for 15 min at 15 psi pres-
sure–121°C.
Preparation of Medium: Add components, ... or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use: For the cultivation of fungi.
Fungi Kimmig Agar
Composition per liter:
Glucose 19.0g
Agar 15. 0g
Peptone 15. 0g
NaCl...
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