Handbook of Microbiological Media, Fourth Edition part 12 pps

... pressure 121 °C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of a variety of anaerobic microorganisms. With added blood for the detection of hemolytic activity of clostridia, streptococci, ... pressure 121 °C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of a variety of anaerobic microorganisms. With added blood for the...

Ngày tải lên: 03/07/2014, 18:20

... examination of water and food. Sources of Media The Handbook of Microbiological Media includes the media produced by major suppliers of dehydrated media, including Oxoid Unipath, HiMedia, and ... to cultivate microorganisms. Uses of Media The Handbook of Microbiological Media contains all the media used to cultivate bacteria, archaea, fungi, and protists of the Americ...

Ngày tải lên: 03/07/2014, 18:20

... pressure 121 °C. Prior to inoculation of cultures, aseptically and anaerobically add 0.1mL of sterile reducing agent solution and 0.5mL of sterile glucose solution to each tube containing 9.4mL of sterile ... tubes. Use: For the maintenance of cultures of Escherichia coli and Staphy- lococcus aureus. For the detection of antibacterial activity of fabrics. Test cultures of Es...

Ngày tải lên: 03/07/2014, 18:20

... pressure– 121 °C. Aseptically add 0.5mL of sterile L-cysteine solution. Mix thor- oughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of a variety of fungi. Acidaminobacter ... 1.0g KAl(SO 4 ) 2 ·12H 2 O 0.18g CuSO 4 ·5H 2 O 0.1g H 3 BO 3 0.1g Na 2 MoO 4 ·2H 2 O 0.1g Na 2 SeO 4 0.1g Na 2 WO 4 ·2H 2 O 0.1g Preparation of Wolfe’s Mineral Elixir: Adj...

Ngày tải lên: 03/07/2014, 18:20

... and bring to boiling for 1 min. Autoclave for 15 min at 15 psi pressure 121 °C. Cool to 50°–55°C. Add 4.0mL of a 10% solution of L-cysteine·HCl·H 2 O that has been filter sterilized. Mix thoroughly. Pour ... boiling for 1 min. Au- toclave for 15 min at 15 psi pressure 121 °C. Cool to 50°–55°C. Add 4.0mL of L-cysteine·HCl·H 2 O solution and 10.0mL of sterile antibiotic solution. Mix t...

Ngày tải lên: 03/07/2014, 18:20

... 15 psi pressure 121 °C. Cool to 50°–55°C. Aseptically add 150.0mL of sterile liver extract, 50.0mL of sterile horse blood, 10.0mL of sterile L- cysteine·HCl solution, 10.0 mL of sterile solution ... 15 psi pressure 121 °C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of a variety of microorganisms. For the prep- aration of blood agar by the additio...

Ngày tải lên: 03/07/2014, 18:20

... 15 min at 15 psi pressure 121 °C. Use: For the detection of E. coli and coliforms. Bile and Brilliant Green extensively inhibit the growth of accompanying flora, in partic- ular Gram-positive ... Durham tube. Autoclave for 10 min at 15 psi pressure 121 °C. Use: For the differentiation of a variety of microorganisms based on their fermentation of specific carbohydrates. Bacteria tha...

Ngày tải lên: 03/07/2014, 18:20

... aseptically add 27.0mL of sterile lactose solution, 10.0mL of sterile Bromthymol Blue solution, 2.0mL of sterile sodium thiosulfate solution, 1.2mL of sterile glucose so- lution, and 1.0mL of sterile maranil ... min at 15 psi pressure 121 °C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile horse serum, 1.0mL of sterile ampicillin solution, 0.25mL of ster- ile urea solution...

Ngày tải lên: 03/07/2014, 18:20

... 25.0g Preparation of Glucose Solution: Add 25.0g of glucose to 50.0mL of distilled/deionized water. Mix thoroughly and gently heat to dissolve. Filter sterilize. Preparation of Medium: Add components, ... medium. Aseptically add 72.0mL of sterile rabbit serum. Use: For the cultivation of a wide variety of microorganisms in a chemically defined medium. For the cultivation of Bo...

Ngày tải lên: 03/07/2014, 18:20

... 15 psi pressure 121 °C. Aseptically add 0.2mL of sterile solution A, 0.2mL of sterile solution B, 0.1mL of sterile solution C, and 0.1mL of sterile so- lution D for each 10.0mL of medium. Mix thoroughly. ... water. Mix thoroughly. Vitamin B 12 Solution: Composition per 100.0mL: Vitamin B 12 (cyanocobalamin) 2.0mg Preparation of Vitamin B 12 Solution: Add vitamin B 12...

Ngày tải lên: 03/07/2014, 18:20