Handbook of Microbiological Media, Fourth Edition part 10 ppt

... min with magnetic stirring. Add 860.0mL of solution B, 5.0mL of solution C, 5.0mL of solution D, 100 .0mL of solution E, and 20.0mL of solution F through one of the screw-cappped openings under 95% ... 5.2g Preparation of A & A FeEDTA Solution: Dissolve 5.2g of KOH in 186.0mL of distilled/deionized water. Add 20.4g of disodium EDTA·2H 2 O. Add 13.7g of FeSO4·7H2O to...

Ngày tải lên: 03/07/2014, 18:20

... the numbers of particular species of microorganisms. Most often, mixtures of growth factors are used in microbiological media. Acid hydrolysates of casein commonly are used as sources of amino acids. ... 1924 Z 1953 INDEX 1957 © 2 010 by Taylor and Francis Group, LLC Handbook of MICROBIOLOGICAL MEDIA Fourth Edition Ronald M. Atlas Washington, D.C. CRC Press is an imprin...

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... concentration of the heat-killed bacteria to 10 10 cells per mL. As a check that the cells are not viable, add 3 drops of the cell suspension prepared in step 10 to the edge of a 100 .0mm Pe- tri ... anaerobically add 10. 0mL NaHCO 3 solution, 10. 0mL butanediol solution, 10. 0mL Na 2 S·9H 2 O solution, 10. 0mL galactose soltuion, 10. 0mL yeast extract solution, and 1.0mL tra...

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... per 100 .0mL: Urea 20.0g Preparation of Urea Solution: Add urea to distilled/deionized wa- ter and bring volume to 100 .0mL. Mix thoroughly. Filter sterilize. Warm to 50°–55°C. Preparation of Medium: ... solution 10. 0mL pH 7.2 ± 0.2 at 25°C Glucose Solution: Composition per 10. 0mL: Glucose 2.0g Preparation of Glucose Solution: Add glucose to distilled/deion- ized water and bring...

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... cultivation of a wide variety of bacteria. BAGG Broth (Buffered Azide Glucose Glycerol Broth) Composition per liter: Pancreatic digest of casein 10. 0g Peptic digest of animal tissue 10. 0g Glucose ... Enrichment: Composition per 100 .0mL : Chicken egg yolks 10 K 2 TeO 3 0.15g NaCl (0.9% solution) 50.0mL Preparation of Egg Yolk Tellurite Enrichment: Soak eggs with 1 :100 dil...

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... extract 10. 0g Starch 10. 0g NaCl 5.0g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g Charcoal 4.0g Nicotinic acid 1.0mg Preparation of Charcoal Agar Base: Add components of char- coal ... per 100 .0mL: Bromthymol Blue 0.5g Ethanol, 95% 100 .0mL Preparation of Bromthymol Blue Solution: Add Bromthymol Blue to 100 .0mL of 95% ethanol. Mix thorougly. Vitam...

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... Bromthymol Blue and Cresol Red to 100 .0mL of ethanol. Mix thoroughly. Solution 2: Composition per 100 .0mL: Cellobiose 10. 0g Colistin 400,000U Polymyxin B 100 ,000U Preparation of Solution 2: Add cellobiose ... Sparge under 100 % N 2 for 10 min. Autoclave under 100 % N 2 for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 50.0mL of ster- ile cellobiose...

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... psi pres- sure–121°C. Cool to 45°–50°C. Preparation of Medium: Combine 1.0L of Columbia blood agar base with 9.0g of soluble starch and 10. 0μg of resazurin. Mix thor- oughly. Gently heat and bring ... 0.05g ZnSO 4 ·7H 2 O 70.0μg H 3 BO 3 10. 0μg MnSO 4 ·5H 2 O 10. 0μg Na 2 MoO 4 ·2H 2 O 10. 0μg CoCl 2 ·6H 2 O 5.0μg CuSO 4 ·5H 2 O 5.0μg FeCl 3 ·6H 2 O 1.0mg Trimethylamine soluti...

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... of sterile solution A with 1.0mL of sterile solution B, 100 .0mL of sterile solution C, 10. 0mL of sterile solution D, 10. 0mL of sterile solution E, and 10. 0mL of sterile solution F, in that order. ... 1.0L of sterile solution A, add in or- der: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of sterile solution D, 10. 0mL of sterile solution E, 1...

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... combine 870.0mL of sterile solution A with 1.0mL of sterile solution B, 100 .0mL of sterile solution C, 10. 0mL of sterile solution D, 10. 0mL of sterile solution E, and 10. 0mL of sterile solution ... chloride solution 10. 0mL Magnesium chloride solution 10. 0mL Yeast extract solution 10. 0mL Lactate solution 10. 0mL Sulfite solution 10. 0mL Na 2 S·9H 2 O solution 10. 0...

Ngày tải lên: 03/07/2014, 18:20