Handbook of Microbiological Media, Fourth Edition part 9 pot

... of Medium: Aseptically combine 90 0.0mL of solu- tion A with 100.0mL of solution B. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of ... 500.0mL of solu- tion A with 500.0mL of solution B. Mix thoroughly. Bring pH to 9. 5. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and mai...

Ngày tải lên: 03/07/2014, 18:20

... solution 10.0mL Na 2 S·9H 2 O solution 10.0mL pH 8.2 ± 0.2 at 25°C Na 2 S·9H 2 O Solution: Composition per 10.0mL: Na 2 S·9H 2 O 0.5g Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis- tilled/deionized ... solution 10.0mL Na 2 S·9H 2 O solution 10.0mL pH 8.2 ± 0.2 at 25°C Na 2 S·9H 2 O Solution: Composition per 10.0mL: Na 2 S·9H 2 O 0.5g Preparation of Na 2 S·9H 2 O Solution...

Ngày tải lên: 03/07/2014, 18:20

... bring volume to 99 9.9mL. Mix thor- oughly. Gently heat and bring to boiling. Adjust pH to 6.6. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 0.1mL of sterile biotin ... thoroughly. Filter ster- ilize. Preparation of Medium: Add components, except vitamin solu- tion, to distilled/deionized water and bring volume to 99 9.0mL. Mix thoroughly. Adjust pH t...

Ngày tải lên: 03/07/2014, 18:20

... 15 min at 15 psi pressure–121°C. Na 2 S·9H 2 O Solution: Composition per 10.0mL: Na 2 S·9H 2 O 0.5g Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis- tilled/deionized water and ... Salts: Composition per liter: MgSO 4 ·7H 2 O 29. 7g Nitrilotriacetic acid 10.0g CaCl 2 ·2H 2 O 3.34g FeSO 4 ·7H 2 O 99 .0mg Ammonium molybdate 9. 25mg Metals “44” 50.0mL Preparation of Modifi...

Ngày tải lên: 03/07/2014, 18:20

... 1.0g Fe 4 (P 2 O 7 ) 3 ·9H 2 O solution 10.0mL pH 6 .9 ± 0.2 at 25°C Fe 4 (P 2 O 7 ) 3 ·9H 2 O Solution: Composition per 10.0mL: Fe 4 (P 2 O 7 ) 3 ·9H 2 O 0.25g Preparation of Fe 4 (P 2 O 7 ) 3 ·9H 2 O Solution: ... liter: H 3 BO 3 2.86g MnCl 2 ·4H 2 O 1.81g Na 2 MoO 4 ·2H 2 O 0.39g ZnSO 4 ·7H 2 O 0.222g CuSO 4 ·5H 2 O 0.079g Co(NO 3 ) 2 ·6H 2 O 0.049g Preparation of Trace Metal Mix A5...

Ngày tải lên: 03/07/2014, 18:20

... 45°–50°C. Aseptically add 100.0mL of sterile asparagine solution, 100.0mL of ster- ile methionine solution, 100.0mL of sterile sorbose solution, 10.0mL of sterile yeast extract solution, 10.0mL of sterile sodium ... 10.0mL: Irgasan 0.04g Ethanol (95 % solution) 10.0mL Preparation of Irgasan Solution: Add Irgasan to 10.0mL of etha- nol. Mix thoroughly. Filter sterilize. Preparat...

Ngày tải lên: 03/07/2014, 18:20

... isolation of various bacteria; with the addition of blood, for the cultivation of fastidious bacteria. For the cul- tivation of Bordetella pertussis for vaccine production and the mainte- nance of stock ... isolation of various bacteria; with the addition of blood, for the cultivation of fastidious bacteria. For the cul- tivation of Bordetella pertussis for vaccine productio...

Ngày tải lên: 03/07/2014, 18:20

... 7.0mL into tubes that contain meat particles (1 part meat parti- cles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C. Use: For the cultivation of various anaerobes. Chopped Meat ... 7.0mL into tubes that contain meat particles (1 part meat particles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C. Use: For the cultivation of numerous anaerobes, inclu...

Ngày tải lên: 03/07/2014, 18:20

... 1.04g KH 2 PO 4 0.68g Na 2 S·9H 2 O solution 0.15g pH 7.0 ± 0.2 at 25°C Na 2 S·9H 2 O Solution: Composition per 10.0mL: Na 2 S·9H 2 O 0.3g Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis- tilled/deionized ... pressure–121°C. Preparation of Medium: To each tube containing 8.9mL of sterile solution A, add (using a syringe) 1.0mL of sterile solution B, 0.1mL of steril...

Ngày tải lên: 03/07/2014, 18:20

... cells L9 29 (ACC 2) or HeLa (ACC 57) cells. Incubate at 37°C plus 5% CO 2 . When a confluent layer has formed, infection can be carried out. Exchange medium with 6.0mL of IM with the addition of ... 20.0g Pancreatic digest of casein 20.0g MgSO 4 ·7H 2 O 2.0g Potassium phosphate buffer (0.02M solution, pH 7.6) 500.0mL pH 7.6 ± 0.2 at 25°C Preparation of Medium: Add agar, pancreatic d...

Ngày tải lên: 03/07/2014, 18:20