Handbook of Microbiological Media, Fourth Edition part 8 docx

... 10.0mL of CaCl 2 solution, 10.0mL of MgSO 4 ·7H 2 O solution, 10.0mL of NaNO 3 solution, 10.0mL of K 2 HPO 4 solution, 10.0mL of KH 2 PO 4 solution, and 10.0mL of NaCl solution to distilled/deionized water ... Base: Composition per 165.0mL: Pancreatic digest of casein 2.72g Agar 2.1g NaCl 0.8g Papaic digest of soybean meal 0.48g K 2 HPO 4 0.4g Glucose 0.4g Preparation o...

Ngày tải lên: 03/07/2014, 18:20

... determination of phenol coefficients of disinfectant prod- ucts containing cationic surface-active materials. Use according to Official Methods of Analysis of the Association of Official Analytical Chemists ... inoculation of cultures, add 0.25mL of sterile Na 2 S·9H 2 O solution to each tube containing 9.75mL of sterile basal medium. Use: For the cultivation and maintenance...

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... tubes. Use: For the cultivation of Azospirillum lipoferum. Azospirillum lipoferum Agar Medium Composition per liter: Agar 15.0g Calcium malate 10.0g K 2 HPO 4 0.8g MgSO 4 ·7H 2 O 0.5g KH 2 PO 4 ... 0.22g CaSO 4 ·2H 2 O 0.1g MgSO 4 ·7H 2 O 0.098g NaCl 0.058g K 2 HPO 4 0.058g FeSO 4 ·7H 2 O 5.0mg Na 2 MoO 4 ·2H 2 O 0.2mg pH 7.2 ± 0.2 at 25°C Preparation of Medium: Add components to disti...

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... Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). Water-lysed blood may be omitted if sterile blood is partially lysed due ... 10.0mL of sterile CaCl 2 ·2H 2 O solution, 10.0mL of sterile MgSO 4 ·7H 2 O solution, 10.0mL of sterile sodium ac- etate solution, 2.0mL of sterile yeast extract solution, and...

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... 15.0mL Preparation of Part B: Prepare a 1M solution of K 2 CO 3 and filter sterilize. Filter sterilize 25.0mL of 95% ethanol solution. Aseptically combine 20.0mL of sterile K 2 CO 3 solution and 15.0mL of ... medium under 80 % N 2 + 20% CO 2 . Anaerobically combine 900.0mL of cooled solution A with 80 .0mL of sparged solution B. Mix thoroughly. Adjust pH to 8. 3. Anaerob...

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... maintenance of a wide variety of microor- ganisms. Dextrose Ascitic Fluid Semisolid Agar Composition per liter: Pancreatic digest of casein 2.66g NaCl 1.33g Agar 0.5g Phenol Red 4.8mg Ascitic ... and maintenance of a wide variety of microor- ganisms. © 2010 by Taylor and Francis Group, LLC Dey-Engley Neutralizing Agar 589 Use: For the isolation, cultivation, and enumeration of “f...

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... Medium: To 80 .0mL of cooled, sterile basal agar, aseptically add 20.0mL of sterile horse serum, 1.0mL of sterile fresh yeast extract solution, 1.0mL of sterile urea solution, 0.5mL of sterile CVA ... 3×: Composition per liter: NaCl 70.43g MgCl 2 ·6H 2 O 31 .86 g Na 2 SO 4 11.75g CaCl 2 ·2H 2 O 4.35g NaHCO 3 2 .88 g KCl 1.99g KBr 0.29g H 3 BO 3 0.08g Preparation of Artificial...

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... Solution: Composition per liter: H 3 BO 3 2 .86 0 MnCl 2 ·4H 2 O 1 .81 g ZnSO 4 ·7H 2 O 0.22g CuSO 4 ·5H 2 O 0.08g Na 2 MoO 4 ·4H 2 O 0.025g CoCl 2 0.025g Preparation of Trace Elements Solution: Add components ... of Solution C: Add NaHCO 3 to distilled/deionized water and bring volume to 14.0mL. Mix thoroughly. Sparge with 80 % N 2 + 20% CO 2 . Filter sterilize. Preparation of Me...

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... pressure–121°C. Preparation of Medium: To each tube containing 8. 9mL of sterile solution A, add (using a syringe) 1.0mL of sterile solution B, 0.1mL of sterile solution C, and 0.01mL of sterile solution ... maintaining at 80 ° 85 °C for 20 min after the addition of sheep blood. Use: For the cultivation and maintenance of fastidious microorgan- isms. For the cultivation and ma...

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... 300.0mL of sterile solution B and 200.0mL of sterile solution C. Mix thoroughly. Aseptically dispense into sterile tubes or flasks. Use: For the general cultivation of a wide variety of bacteria. FSM ... add 50.0mL of sterile bovine serum and 10.0mL of sterile streptomycin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the culti...

Ngày tải lên: 03/07/2014, 18:20