... Preparation of Medium: To 916.0mL of sterile solution A, add 70 .0mL of sterile solution B, 10.0mL of sterile solution C, 10.0mL of sterile solution D, and 10.0mL of sterile solution E. Mix thoroughly.Anaerobically ... Bring pH to 7. 0 with20% NaOH. Adjust pH to 7. 2 with HCl. Do not autoclave. Add 0.25mL of chloroform and store at 4°C. Before use, heat to 56°C to removechloroform. Preparation of Medium: Add ... 100.0mL:ZnSO4·7H2O 1.095gFeSO4·7H2O 0.5gSodium EDTA 0.25gMnSO4·H2O 0.154gCuSO4·5H2O 39.2mgCo(NO3)2·6H2O 24.8mgNa2B4O 7 ·10H2O 17. 7mgPreparation of “Metals 44”:...