Handbook of Microbiological Media, Fourth Edition part 7 potx

... 3.0g Pancreatic digest of casein 1.7g Tween™ 80 1.0g NaCl 0.5g Papaic digest of soybean meal 0.3g K 2 HPO 4 0.25g Bromcresol Purple 0.06g pH 7. 8 ± 0.2 at 25°C Preparation of Medium: Add components ... liter: Dextrin 15.0g Agar 13.0g Pancreatic digest of casein 10.0g Na 2 HPO 4 7. 75g NaCl 5.0g Beef extract 3.0g Na 2 SO 3 1.6g Acid Fuchsin solution 50.0mL pH 7. 5 ± 0.2 at 25°C A...

Ngày tải lên: 03/07/2014, 18:20

... per 100.0mL: CaCl 2 ·2H 2 O 1.457g FeSO 4 ·7H 2 O 0.366g ZnSO 4 ·7H 2 O 0. 178 g MnSO 4 ·H 2 O 0.101g Na 2 MoO 4 ·2H 2 O 23.4mg CuSO 4 ·5H 2 O 7. 8mg Preparation of Trace Elements Solution: Add ... per 100.0mL: CaCl 2 ·2H 2 O 1.457g FeSO 4 ·7H 2 O 0.366g ZnSO 4 ·7H 2 O 0. 178 g MnSO 4 ·H 2 O 0.101g Na 2 MoO 4 ·2H 2 O 23.4mg CuSO 4 ·5H 2 O 7. 8mg Preparation of Trace Elements Solution: A...

Ngày tải lên: 03/07/2014, 18:20

... thoroughly. Dis- tribute 7. 0mL into tubes that contain meat particles (1 part meat parti- cles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C. Use: For the cultivation of various anaerobes. Chopped ... thoroughly. Distribute 7. 0mL into tubes that contain meat particles (1 part meat particles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C. Use: For...

Ngày tải lên: 03/07/2014, 18:20

... 20.0g Pancreatic digest of casein 20.0g MgSO 4 ·7H 2 O 2.0g Potassium phosphate buffer (0.02M solution, pH 7. 6) 500.0mL pH 7. 6 ± 0.2 at 25°C Preparation of Medium: Add agar, pancreatic digest of casein, and MgSO 4 ·7H 2 O ... solution 10.0mL pH 7. 6 ± 0.2 at 25°C Magnesium Sulfate Solution: Composition per 10.0mL: MgSO 4 ·7H 2 O 2.0g Preparation of Magnesium Sulfate Solution: Add...

Ngày tải lên: 03/07/2014, 18:20

... Preparation of Medium: To 916.0mL of sterile solution A, add 70 .0mL of sterile solution B, 10.0mL of sterile solution C, 10.0mL of sterile solution D, and 10.0mL of sterile solution E. Mix thoroughly. Anaerobically ... Bring pH to 7. 0 with 20% NaOH. Adjust pH to 7. 2 with HCl. Do not autoclave. Add 0.25 mL of chloroform and store at 4°C. Before use, heat to 56°C to remove...

Ngày tải lên: 03/07/2014, 18:20

... agar, diphasic base medium 70 0.0mL Rabbit blood, defibrinated 300.0mL Locke’s solution 150.0mL pH 7. 2 7. 4 at 25°C Blood Agar, Diphasic Base Medium: Composition per 75 0.0mL: Beef 25.0g Agar 10.0g Neopeptone ... per liter: NH 4 Cl 2.0g Na 2 HPO 4 0.21g MgSO 4 ·7H 2 O 0.2g NaH 2 PO 4 0.09g KCl 0.04g CaCl 2 0.015g FeSO 4 ·7H 2 O 1.0mg ZnSO 4 ·7H 2 O 0.07mg H 3 BO 3 0.01mg MnSO 4 ·5H 2...

Ngày tải lên: 03/07/2014, 18:20

... the cultivation of Escherichia coli. LB Top Agar Composition per liter: Pancreatic digest of casein 10.0g Agar 7. 0g NaCl 5.0g Yeast extract 5.0g pH 7. 0 ± 0.2 at 25°C Preparation of Medium: Add ... 0.5g K 2 HPO 4 0.5g KH 2 PO 4 0.5g MgSO 4 ·7H 2 O 0.2g FeSO 4 ·7H 2 O 0.01g MnSO 4 ·7H 2 O 0.01g NaCl 0.01g Bromcresol Green 0.022g Cycloheximide 7. 0mg pH 6 .7 ± 0.2 at 25°C © 2010 b...

Ngày tải lên: 03/07/2014, 18:20

... 0.5g CaCl 2 ·2H 2 O 0.07g MnSO 4 ·H 2 O 0.017g pH 7. 3 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7. 3. Dis- tribute ... volume to 1.0L. Mix thoroughly. Preparation of Medium: Add 10.0g of pancreatic digest of casein to 1.0L of synthetic seawater. Mix thoroughly. Adjust pH to 7. 8. Distr...

Ngày tải lên: 03/07/2014, 18:20

... aseptically dilute 200.0mL of 5× stock solution with 78 9.0mL of sterile distilled/deionized water. Aseptically add 10.0mL of sterile carbohydrate solution and 1.0mL of sterile MgSO 4 ·7H 2 O solution. ... 100.0mL: ZnSO 4 ·7H 2 O 1.095g FeSO 4 ·7H 2 O 0.5g Sodium EDTA 0.25g MnSO 4 ·H2O 0.154g CuSO 4 ·5H 2 O 39.2mg Co(NO 3 ) 2 ·6H 2 O 24.8mg Na 2 B 4 O 7 ·10H 2 O 17. 7mg Preparation...

Ngày tải lên: 03/07/2014, 18:20

... anaerobically add 60.0mL of sterile NaHCO 3 solution, 5.0mL of sterile 2-propanol, 3.0mL of sterile Na 2 S·9H 2 O solution, 1.0mL of sterile cyanocobalamin solution, 1.0mL of sterile selenite-molybdate-tungstate ... Solution: Composition per liter: MgSO 4 ·7H 2 O 3.0g Nitrilotriacetic acid 1.5g NaCl 1.0g MnSO 4 ·2H 2 O 0.5g CoSO 4 ·7H 2 O 0.18g ZnSO 4 ·7H 2 O 0.18g CaCl 2 ·2H 2 O...

Ngày tải lên: 03/07/2014, 18:20