Handbook of Microbiological Media, Fourth Edition part 3 pps

... dinucleotide 0.25g Cocarboxylase 0.1g Guanine·HCl 0.03g Fe(NO 3 ) 3 0.02g p-Aminobenzoic acid 0.013g Vitamin B 12 0.01g Thiamine·HCl 3. 0mg Preparation of CVA Enrichment: Add components to distilled/ deionized ... 0.02g CuSO 4 ·5H 2 O 0.01g H 3 BO 3 0.01g Na 2 MoO 4 ·2H 2 O 0.01g Na 2 SeO 3 ·5H 2 O 0.3mg Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic acid to 500...

Ngày tải lên: 03/07/2014, 18:20

... examination of water and food. Sources of Media The Handbook of Microbiological Media includes the media produced by major suppliers of dehydrated media, including Oxoid Unipath, HiMedia, and ... dinucleotide 0.25g Cocarboxylase 0.1g Guanine·HCl 0.03g Fe(NO 3 ) 3 0.02g Vitamin B 12 0.01g p-Aminobenzoic acid 0.013g Thiamine·HCl 3. 0mg Preparation of CVA Enrichment: Add co...

Ngày tải lên: 03/07/2014, 18:20

... cultivation of Acidobacterium capsulatum. Acidolobus aceticus Medium (DSMZ Medium 901) Composition per 1055mL: Sulfur, powdered 10.0g NH 4 Cl 0 .33 g KCl 0 .33 g KH 2 PO 4 0 .33 g MgCl 2 ·6H 2 O 0 .33 g CaCl 2 ·2H 2 O ... Solution: Composition per liter: H 3 BO 3 0.3g CoCl 2 ·6H 2 O 0.2g ZnSO 4 ·7H 2 O 0.1g MnCl 2 ·4H 2 O 0.03g Na 2 MoO 4 ·2H 2 O 0.03g NiCl 2 ·6H 2 O 0.02g CuCl 2 ·2H...

Ngày tải lên: 03/07/2014, 18:20

... 0.025g KAl(SO 4 ) 2 ·12H 2 O 0.02g H 3 BO 3 0.01g Na 2 MoO 4 ·4H 2 O 0.01g CuSO 4 ·5H 2 O 0.01g Na 2 SeO 3 ·5H 2 O 0.3mg Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized ... 0.02g CuSO 4 ·5H 2 O 0.01g H 3 BO 3 0.01g Na 2 MoO 4 ·2H 2 O 0.01g Na 2 SeO 3 ·5H 2 O 0.3mg Preparation of Wolfe’s Mineral Solution: Add nitrilotriaceti...

Ngày tải lên: 03/07/2014, 18:20

... sterilize. Fe 4 (P 2 O 7 ) 3 ·9H 2 O Solution: Composition per 10.0mL: Fe 4 (P 2 O 7 ) 3 ·9H 2 O 0.25g Preparation of Fe 4 (P 2 O 7 ) 3 ·9H 2 O Solution: Add Fe 4 (P 2 O 7 ) 3 ·9H 2 O to distilled/deionized ... sterilize. Fe 4 (P 2 O 7 ) 3 ·9H 2 O Solution: Composition per 10.0mL: Fe 4 (P 2 O 7 ) 3 ·9H 2 O 0.25g Preparation of Fe 4 (P 2 O 7 ) 3 ·9H 2 O Solution: Add Fe 4...

Ngày tải lên: 03/07/2014, 18:20

... 5.0g Na 2 SO 3 3. 24g CaCl 2 1.8g Yeast extract 1.0g KCl 0.55g NaHCO 3 0.16g Ferric citrate 0.1g KBr 0.08g SrCl 2 0.03g H 3 BO 3 0.02g Na 2 HPO 4 8.0mg Na 2 SiO 3 4.0mg NaF 2.4mg NH 4 NO 3 1.6mg Biotin ... A5: Composition per liter: H 3 BO 3 2.86g MnCl 2 ·4H 2 O 1.81g Na 2 MoO 4 ·2H 2 O 0 .39 g ZnSO 4 ·7H 2 O 0.222g CuSO 4 ·5H 2 O 0.079g Co(NO 3 ) 2 ·6H 2 O 0.049g Prepa...

Ngày tải lên: 03/07/2014, 18:20

... Addition of 1.0g of so- dium sulfacetamide and 250.0mg of sodium mandelate enhances inhi- bition of contaminating microorganisms. Pour into sterile Petri dishes. Use: For the selective isolation of ... distilled/deionized water. The pH of this solution should be 7.0–7.5. Mix thoroughly. Bring volume to 975.0mL with distilled/deionized water. Adjust pH to 7.4. Add 13. 3mL of 0.1%...

Ngày tải lên: 03/07/2014, 18:20

... aseptically add 27.0mL of sterile lactose solution, 10.0mL of sterile Bromthymol Blue solution, 2.0mL of sterile sodium thiosulfate solution, 1.2mL of sterile glucose so- lution, and 1.0mL of sterile maranil ... and cultivation of members of the Enterobacteriaceae. Bromthymol Blue Broth Composition per 101.45mL: Pancreatic digest of casein 0.7g NaCl 0.5g Beef extract 0.3g Yea...

Ngày tải lên: 03/07/2014, 18:20

... 0.02g CuSO 4 ·5H 2 O 0.01g H 3 BO 3 0.01g Na 2 MoO 4 ·2H 2 O 0.01g Na 2 SeO 3 ·5H 2 O 0.3mg Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. ... 1.0mL pH 7.2–7 .3 ± 0.2 at 25°C Vitamin Solution: Composition per 50.0mL: Thiamine·HCl 8 43. 3mg Pantothenic acid 595.8mg Nicotinic acid 30 7.8mg p-Aminobenzoic acid 68.6mg Pyrid...

Ngày tải lên: 03/07/2014, 18:20

... 60.0mL of sterile Na 2 S·9H 2 O solution, 40.0mL of ster- ile NaHCO 3 solution, and 1.0mL of sterile vitamin B 12 solution. Mix thoroughly. Adjust pH to 7 .3 with sterile H 2 SO 4 or Na 2 CO 3 . ... 0.5mg NaHCO 3 solution 20.0mL Na 2 S·9H 2 O solution 20.0mL Trace elements solution 1.0mL pH 7.0 ± 0.2 at 25°C NaHCO 3 Solution: Composition per 20.0mL: NaHCO 3 2.0g Preparati...

Ngày tải lên: 03/07/2014, 18:20