... reaction rate with [deoxyHb] and [NO À 2 ]. This gave values of 2.5 and 1.0 m )1 Æs )1 for carp Hb in the absence and presence of ATP, respec- tively, and 0.06 m )1 Æs )1 for rabbit Hb, which illustrates the ... saturations. Initial oxygen saturations (S O 2 ) were: (A) 2%, (B) 28%, (C) 46%, (D) 67% and (E) 100%. The hemoglobin concentration was 155 l M, and th...
Ngày tải lên: 16/03/2014, 06:20
... project for the morphological and syntactic layers, the LE-SIMPLE project for the semantic model and lexicon and the Italian project CLIPS 2 for the phonological level and the extension of the ... results of the EWN and ACQUILEX projects and on a revised version of Pustejovsky’s Generative Lexicon theory (Pustejovsky 199 5). In spite of the different underlying principl...
Ngày tải lên: 17/03/2014, 04:20
Báo cáo Y học: Propionate CoA-transferase from Clostridium propionicum Cloning of the gene and identi®cation of glutamate 324 at the active site pdf
... faint bands at 40 kDa and 20 kDa in lanes 2±4 which are absent in lane 5. Cell free extract (lane 1, 50 lg), Q-Sepharose (lane 2, 10 lg), Resource-Phe (lane 3, 5 lg) , Resource-Q (lane 4, 5 lg). ... )1 35 kJ ámol )1 acetate. The enzymes in volved are p yru- vate:glutamate transaminase ( 1), g lutamate dehydrogenase ( 2) (R)-lactate dehydrogenase ( 3), propionate C oA-transfe...
Ngày tải lên: 17/03/2014, 17:20
Báo cáo khoa học: Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells pot
... was measured at 5 days. (D) Effect of IxoA on SW480 cells at 1, 2, 4, 5, 6 and 7 days. The experimental procedures for (B), (C) and (D) were the same as in (A). Tomatillo and colon carcinogenesis ... USA). Rabbit polyclonal DP-1 (clone K-2 0) sc-610 antibody and goat polyclonal b-actin (clone I-1 9) antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). C...
Ngày tải lên: 23/03/2014, 10:20
Báo cáo khoa học: "Extracting Noun Phrases from Large-Scale Texts: A Hybrid Approach and Its Automatic Evaluation" pot
... 1.0/(1.0+ABS(Iog(P(. )) )) o/w. We then rewrite ( 6) as ( 8). ( 8) C* = argmaxP(C, It," ;) n~ -= argmaxI- I P,(q [c._ ,) x P, (c .) f=l = argmax Z [log(P~ (c, Ic,_, )) + log(P~ (c ,)) l k=l r~ = ... positive, 2) large value means high score, and 3) the scores are between 0 and 1, we define a score function S(P( • )) shown as ( 7). (...
Ngày tải lên: 31/03/2014, 06:20
Tài liệu Báo cáo khoa học: The multicopper oxidase from the archaeon Pyrobaculum aerophilum shows nitrous oxide reductase activity docx
... assays with McoP, and ABTS in reactions using CotA laccase. Enzyme Substrates K m (lM) k cat (s )1 ) k cat ⁄ K m (M )1 Æs )1 ) McoP Fe 2+ ⁄ O 2 O 2 31 ± 0.2 3 ± 0.2 0.9 · 10 5 Fe(II) 35 ± 0.2 0.9 ... Fernandes et al. 3186 FEBS Journal 277 (201 0) 3176–3189 ª 2010 The Authors Journal compilation ª 2010 FEBS cloramphenicol (34 lgÆmL )1 ), arabinose (1 mgÆmL )1...
Ngày tải lên: 18/02/2014, 04:20
Tài liệu Báo cáo khoa học: Enzyme kinetics informatics: from instrument to browser pdf
... EPSRC grant BB ⁄ C008219 ⁄ 1, and the Klaus Tschira Founda- tion (KTF) and the German Federal Ministry of Edu- cation and Research (BMBF) for funding the Scientific Databases and Visualization group ... (200 7) Storing and annotat- ing of kinetic data. In Silico Biol 7(Suppl 2), S37–44. 24 Kell DB. (200 6) Metabolomics, modelling and machine learning in systems biology: tow...
Ngày tải lên: 18/02/2014, 04:20
Tài liệu Báo cáo khoa học: Multisite protein phosphorylation – from molecular mechanisms to kinetic models pdf
... kinase II (CaMKII) RXX(S ⁄ T)– – Casein kinase 1 (CK 1) (Sp/Tp)XX(S ⁄ T) – Primed substrate (D ⁄ E)XX(S ⁄ T)– – Casein kinase 2 (CK 2) (S ⁄ T)XX(Sp ⁄ Tp) – Primed substrate (S ⁄ T)XX(D/E) – – Glycogen ... kinase 3 (GSK 3) (S/T)XXX(Sp ⁄ Tp) – Primed substrate Protein kinase B (PKB ⁄ Akt) RXRXX(S ⁄ T)– – Protein kinase C (PKC) (S ⁄ T)X(K ⁄ R) – – Tyr kinases EGF receptor kinase X(D ⁄ E...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: An alternative transcript from the death-associated protein kinase 1 locus encoding a small protein selectively mediates membrane blebbing pdf
... indicated (wt, TM1, and TD; s-DAPK-1Dtail) and evaluated for membrane blebbing in transfected cells as described previously [10]. The top panel (B) shows the normal ( 1) and blebbing ( 2) morphology. ... procedures Cell culture and harvest, plasmids and transfection, and treatment HEK293 (human embryonic kidney cell line) and A375 (human melanoma) cells were cultured in DM...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: Aldehydes release zinc from proteins. A pathway from oxidative stress⁄lipid peroxidation to cellular functions of zinc pptx
... Meeting, PA), Sephadex G-25 and G-50 from Amersham Biosciences (GE Healthcare, Piscataway, NJ), Cleland’s reagent (dithio- threitol) from Calbiochem (San Diego, CA), and Zinquin ester from Molecular ... isoform 2 (MT 2). MT2 (1.2 l M) was incubated without (line 1) or with (line 2) acetaldehyde (1 m M) for 30 min in 20 mM Tris ⁄ HCl (pH 7. 4), 5,5¢-dithiobis-2-nitrobenzoic...
Ngày tải lên: 19/02/2014, 06:20