... Virol., Vol.70, No .2, 22 8 -23 9. Japan Sewage Works Association. (1997) Wastewater testing method. (in Japanese) Jeng, H.W.C., England, A.J., and Bradford, H.B. (20 05) Indicator organisms associated ... specified in Table 1. The PCR conditions included a denaturing step at 95 oC for 5 min, followed by 40 cycles of 95 oC for 1 min, annealing temperature (Table 1) for 1 min, and 72 oC for 20 ... clay, < 0.005 mm; silt, 0.005 – 0.075 mm; fine sand, 0.075 – 0 .25 mm; medium sand, 0 .25 – 0.85 mm. The samples were collected on May 13th, 20 08. RESULTS AND DISCUSSION Evaluation of recovery...