ADVANCES IN APPLIED BIOTECHNOLOGY pptx

... interfacing zone and this one is connected with the fruit body processing zone by the second interfacing area, as it is shown in figure 11. Advances in Applied Biotechnology 8 The mineral ... achievements in molecular biotechnology and genetic engineering. In the final section, the chapters emphasize the main scientific results that were accomplished in tissue engin...

Ngày tải lên: 28/06/2014, 14:20

... resulting in a substantially increase in recombinant protein yield, the protein yield was still considerably lower than the yield observed in E. coli BL21. This difference in recombinant Increasing ... complex proteins in this host. These engineering attempts are summarized in Fig. 1. Fig. 1. Overview of different engineering approaches to increase recombinant protein prod...

Ngày tải lên: 22/06/2014, 03:20

... inactivate bacterial chromosomal genes (Dabert and Smith, 1997; Advances in Applied Biotechnology 198 pKD46 Host strain pKD46 Host strain Host strain 7. Dual antibiotic selection 6. Incubation5. ... host strain containing the plasmid of pKD46, encoding the recombinase. Electroporation is crucial. • Culturing in the rich medium (SOC) with 1 mM of arabinose, at 37˚C for 2 h....

Ngày tải lên: 22/06/2014, 03:20

... Tissue engineering Advances in cell therapy research gave rise to a fast-growing multidisciplinary field that integrates knowledge of engineering, biology and medicine. Tissue engineering (TE) ... approaches, including in situ coating, post-synthesis adsorption and end- grafting. In fact, several methods have been proposed for their synthesis, coating, and stabilization, mainly comp...

Ngày tải lên: 22/06/2014, 03:20

... first interfacing zone and this one is connected with the fruit body processing zone by the second interfacing area, as it is shown in figure 11. Advances in Applied Biotechnology 12 In ... Cultivation of Edible and Medicinal Mushrooms on Organic Wastes from Wine Making Industry. In: Proceedings of the International Conference „Environmental Engineering and Sustainable D...

Ngày tải lên: 22/06/2014, 03:20

... objective of increasing sustainability in PHAs synthesis processes. 2.2.1 Modification of host strains When they are grown in bioreactors, all microorganisms, including PHB-producing recombinant ... haemoglobin is supposed to facilitate intracellular oxygen transfer and assimilation, and the gene encoding this protein was introduced in PHB-producing E. coli improving the growth an...

Ngày tải lên: 22/06/2014, 03:20

... dealing with the use of bacteriocins or bacteriocin-producing strains for the biopreservation and improving functional properties of vegetable and fruit products. Advances in Applied Biotechnology ... ever-increasing potential of these peptides. Undoubtedly, the most extensively studied bacteriocin is nisin, which has gained widespread applications in the food industry. This F...

Ngày tải lên: 22/06/2014, 03:20

... tyrosine- and putrescine-degrading enzymatic activities of wine- Advances in Applied Biotechnology 80 Although the primary goal is the prevention of mycotoxin contamination, mycotoxin formation ... histamine, tyramine and/or putrescine degradation among wine LAB does not appear to be very frequent, since out of the 85 strains examined, only nine displayed noteworthy amine-degra...

Ngày tải lên: 22/06/2014, 03:20

... Roasting 20min and 40min at 80°C and 120°C Pressurized steam-heating (0.1 MPa, 20 min ; 0.2 MPa, 40 min) Microwaving (700W, 10 min) 20-30% increases depending on the conditions 18-30% increases ... dehulling time (10 to 130 min) leads to greater losses of rutin in the same product grains (Dietrych-Szostak & Oleszek, 1999). Boiling including soaking (100°C/121°C) with/or with...

Ngày tải lên: 22/06/2014, 03:20

... using primers 5 and 4. Advances in Applied Biotechnology 152 3.1 Twin-arginine signal peptide Proteins are targeted to the Tat pathway by tripartite N-terminal signal peptides, the amino- terminal ... harboring Advances in Applied Biotechnology 168 2.1.4 AprX involved in degradation of the α-amylase-A522-PreS2 hybrid protein AprX in the supernatant was able t...

Ngày tải lên: 22/06/2014, 03:20