ecombinant protein production expression systems and animal cell technology

... literature review will outline some aspects of animal cell technology regarding the production of recombinant proteins. PROTEIN EXPRESSION SYSTEMS Recombinant proteins were developed more than 25 years ... RECOMBINANT PROTEIN PRODUCTION: EXPRESSION SYSTEMS AND ANIMAL CELL TECHNOLOGY TÓM TẮT Protein tái tổ hợp đã và đang đƣợc áp dụng rộng rãi trong ... disadvanta...

Ngày tải lên: 28/06/2014, 12:52

... literature review will outline some aspects of animal cell technology regarding the production of recombinant proteins. PROTEIN EXPRESSION SYSTEMS Recombinant proteins were developed more than 25 ... disadvantages and challenges which could suffer from when using animal cell in the production of recombinant proteins.  Due to low yield and complicated and costl...

Ngày tải lên: 28/03/2014, 09:20

... intracellular localizations of the human ER and PDIP46/SKAR proteins We examined the intracellular localization of the human ER and PDIP46 ⁄ SKAR proteins fused with enhanced green fluorescent protein ... nurse cells of the ovaries and in the preblastoderm embryos [1]. After gastrulation, the maternal transcript disappears and the zygotic transcript and protein are found in a sub...

Ngày tải lên: 19/02/2014, 05:20

... gene expression to the same extent in serum- cultured cells and dose-dependently counteracted, and ultimately abolished, the increase in c-myc gene expression elicited by PMA in serum-free cells. ... control of cell proliferation and its overexpression is detected in many tumor cell types [7]. Amplified c-myc expression has also been reported to correlate with metastatic growth...

Ngày tải lên: 21/02/2014, 01:21

... (family member 8), and it interconverts 17b-OH ⁄ 17-oxosteroids [6]. Thus, the activities of the androgen metabolite pair androster- one ⁄ 3,17-androstanediol and abundant expression in steroidogenic ... formation and function, because of its unique tissue distribution [7] and regulated expression [5]. We recently found a new PAT protein, myocardial LD protein, which is highly e...

Ngày tải lên: 07/03/2014, 05:20

... found this technology to be robust, and our wheat germ cell- free pipeline now supports high-throughput screening for protein pro- duction and solubility and provides stable isotope labeled protein ... Se-Met labeling is easier with cell- free systems than with yeast, mammalian, or insect cell systems [3–5]. Cell- free sys- tems may permit successful production of prote...

Ngày tải lên: 07/03/2014, 12:20

... specific subcellular locations where they bind to regulatory and ⁄ or substrate proteins and are integrated into cell signalling pathways and cell cycle events [23]. The UD, Src homology 3 and Src homology ... The proteins were visualized by silver staining, and the protein bands were analysed by MALDI-TOF MS. Protein identification by peptide mass fingerprinting analysis Silve...

Ngày tải lên: 07/03/2014, 12:20

... CIB-myc protein expression, (d±f) coexpression of HA-NBR1 and F EZ1- EGFP, and (g±i) coexpression of HA-NBR1 and CIB-myc. T he pattern of C IB staining observed in (g) w as never observed in cells expressing ... Interactions between proteins NBR1, CIB and FEZ1 (Eur. J. Biochem. 269) 543 NBR1 interacts with fasciculation and elongation protein zeta-1 (FEZ1) and calcium and...

Ngày tải lên: 08/03/2014, 10:20

... these two proteins. To date, several Evi1-binding proteins have been identified using a candidate protein approach. It remains possible that Evi1 interacts with as yet unknown cellular proteins ... Recombinant retrovirus produced in BOSC-23 cells was used to infect Rat1 and RatFL cells and cell populations expressing UXT were selected. Neither UXT expres- sion in Rat1 cells nor empty v...

Ngày tải lên: 16/03/2014, 11:20

... genome. Abbreviations CRABPIa and CRABPIb, cellular retinoic acid-binding protein type I a and b; CRBP, cellular retinol-binding protein; hpf, hours post fertilization; iLBP, intracellular lipid-binding protein; ... crabp1a and crabp1b genes arose from a fish-specific chromosomal duplication Phylogenetic analysis of the mammalian and fish cellu- lar retinoid-binding proteins revealed...

Ngày tải lên: 16/03/2014, 22:20