TARGETS IN GENE THERAPY pptx

... polylysine (K) or a polyarginine (R) tails, based on the Contents Preface IX Part 1 Target Strategy in Gene Therapy 1 Chapter 1 Choosing Targets for Gene Therapy 3 Karina J. ... chronic pseudomonas aeruginosa infections, and dornase alfa use. J Pediatr 153 (6):752-7. Part 1 Target Strategy in Gene Therapy Choosing Targets for Gene Therapy 9...

Ngày tải lên: 28/06/2014, 05:20

... its receptor, trig- gering receptor kinase activity and signaling pathways involving the binding of ezrin to ezrin–radixin–moesin proteins, and thus actin cytoskeleton binding and Ras activation. ... proteins exist in three states with respect to HA binding: non- binding; nonbinding unless activated by physiological stimuli; and constitutively binding [17–19]. HA induces signaling when it b...

Ngày tải lên: 28/03/2014, 23:20

... two- domain IN constructs, namely NTD–CCD and CCD– CTD, are being used as starting points for building models of the complete HIV-1 IN protein and IN DNA complexes [44]. These structures will be informative, ... the IN domains are connected by flexible linkers allowing significant interdomain variability, and a three-domain model may not reflect the actual con- formation(s) of the intact...

Ngày tải lên: 29/03/2014, 23:20

... promotor, resulting in tat- independent transcription. The self-inactivating vector was constructed by deleting 133 bp in the U3 region of the 3¢ LTR including the TATA box and the binding sites for ... deleting retroviral genes and adding gene( s) of interest. Vectors can be produced in pack- aging cell lines that express packaging genes. The major advantage of retroviral vectors is t...

Ngày tải lên: 10/04/2014, 22:11

... of expressing foreign genes in mammalian cells [8,9], in the early 1980s, AAV2 vectors have rapidly gained popularity in gene therapy applications, due to some of their distin- guishing biological ... overcome, rAAV-based gene transfer vectors still rep- resent one of the most promising gene therapy systems and gain increasing popularity. In a search for "AAV gene th...

Ngày tải lên: 18/06/2014, 18:20

... overcome, rAAV-based gene transfer vectors still rep- resent one of the most promising gene therapy systems and gain increasing popularity. In a search for "AAV gene therapy& quot;, 1,016 ... and insertional oncogenesis mediated by the "random" insertion of retroviruses into cellular DNA [48]. Presently, other virus vectors have been gaining an impor- tant place in...

Ngày tải lên: 20/06/2014, 01:20

... accelerated clinical trial of a combination of stavudine/lamivudine/nevirapine in the treatment of people living with HIV-AIDS in Nigeria at the University of Benin teaching hospital, one of the des- ignated ... monitoring of triglyceride and other indices of lipid profile in individuals on HAART, with a view to instituting therapy as early as possible when values are found deranged,...

Ngày tải lên: 20/06/2014, 08:20

... defective host gene remains unaltered. In general, the gene therapy recipient cells may be germline cells or somatic cells. Germline cells therapy involves modifying the genes in germ cells ... Non-Viral Gene Therapy Vectors Carrying Genomic Constructs 9 invasin, from the inv gene of Yersinia pseudotuberculosis, which binds to β1-integrins on mammalian cells leading t...

Ngày tải lên: 27/06/2014, 18:20

... and plays an important role in getting the virus inside a cell. Most of the remaining genes code 18 Gene Therapy CoreFiber Protein VI Capsid DNA genome Terminal protein Penton Hexon Structure of ... terminal proteins. Several other proteins, not shown, are stored in the core to initiate and maintain protection. The product of this gene is a protein called alpha-synuclein, which may...

Ngày tải lên: 29/06/2014, 11:20

... protein kinases Mitogen-activated protein (MAP) kinases are stress-activated serine/threonine kinases that include the p38, ERK (extra- cellular regulating kinase), and JNK (c-Jun-N-terminal kinase) (Figure ... these new kinase inhibitors. Lipid kinases: phosphatidylinositol 3-kinase Several phosphatidylinositol 3-kinase (PI3K) inhibitors have entered clinical trials in different fields, in...

Ngày tải lên: 09/08/2014, 01:22