... mL of 50 mm ammonium acetate, pH 6.0, and loaded onto thecartridge. After washing with 5 mL of 50 mm ammoniumacetate, pH 6.0, the glycans were eluted using 5 mL of 60%acetonitrile in the ammonium ... (Fig. 4). Toreduce fluctuations in retention time, the retention time on size-fractionation HPLC was normalized againstglucose unit, and that on reversed-phase HPLC wasconverted to the reversed-phase ... indicate elution positions of standardPA-N-glycans with 0–4 sialic acids, respectively. (B) Contents of PA-glycans that have PA-GlcNAc at their reducing end in thefractions from anion-exchange...