... temperature for 3 days and continuous illuminated from overhead by using 80W cool-Bristol medium was HPO4 75, KH2PO4 175, O 75, and FeCl2 0 .3, BO 3 0.2, CuSO4.5H2O .0 before sterilization. ... yeast for lipid production. African J Biotechnol 10 ( 63) : 138 67- 138 77. [10] Kwon, D.Y. and Rhee, J.S. (1986) A Simple and rapid colorimetric method for determination of free fatty acids for ... yeast T. maleeae Y30 and lipid yield reached the maximum of 23. 63 g/L and 7.06 g/L were obtained, respectively. Cellular lipid content of 26.8% was obtained. Waste CO2 produced by the fermentation...