... full-length protein. Actu-ally, binding of ibuprofen to the (secondary) tHSAbinding site inhibits Fe(III)heme binding, and, in turn,Fe(III)heme inhibits ibuprofen binding. This findingexplains a former ... ibupro-fen binding to either its primary (in domain III)or secondary (in domain II) binding site in full-lengthHSA. Indeed, after removal of domain III, ibuprofenbinds to a single site, thus allowing ... protein and account for the regulation of ligand-binding modes by heterotropic interactions aremaintained after the removal of domain III. Indeed,warfarin allosterically inhibits Fe(III)heme binding, and, ...