... displayed in Fig. 1. The decay of the flash-induced absorbance of P700+at 820 nm was monoexponential for all proteins at each of the five temperatures (27 8, 28 3, 28 8, 29 3 and 29 8 K). In the range of ... (see the Introduction). Hence, the dependence of k 2 of wild-type and all mutants on the concentration of MgCl 2 was investigated at 27 8, 28 3, 28 8, 29 3 and 29 8 K.Table 1. Kinetic and thermodynamic ... k 2 at in nite ionic strength.(Received 10 June 20 02, revised 5 September 20 02, accepted 15 October 20 02) Eur. J. Biochem. 26 9, 5893–59 02 (20 02) Ó FEBS 20 02 doi:10.1046/j.14 32- 1033 .20 02. 03314.x 25 ....