Báo cáo toán học: "Heterologous expression and optimization using experimental designs allowed highly efficient production of the PHY US417 phytase in Bacillus subtilis 168" ppt
Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii ppt
... allowing the interaction of Antarctic b-tubulin isotypes with CCT in the adverse energetic conditions of the Antarctic habitat. The role of these amino acid substitutions in the folding process of E. ... analysis of the interaction between CCT and b-tubulin by cryoelec- tron microscopy allowed the identification of three regions from the N-terminal, and fiv...
Ngày tải lên: 23/03/2014, 21:20
Báo cáo khoa học: Heterologous expression of a serine carboxypeptidase-like acyltransferase and characterization of the kinetic mechanism potx
... predicted to be mainly involved in l-malate recognition and binding resulted in strong reduction of enzyme activity. The inhibition of SMT catalysis by d-malate reveals the positioning of the reactive ... (Fig. 3). The in vitro kinetics of SMT was examined by assaying the conversion of 1-O-sinapoyl-b-glucose (sinapoylglucose = singlc) to 1-O-sinapoyl-l-malate Heter...
Ngày tải lên: 23/03/2014, 07:20
Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
... ACMSD of the region surrounding the metal active site and the Arg47 likely involved in substrate binding. Discussion In this study, the expression of the human ACMSD transcript coding for the active ... in the brain of a murine model of the syndrome [18,19]. Interestingly, picolinate is reportedly able to prevent the neurotoxic effects of quinolinate in...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx
... result of the lost Ca 2+ ⁄ CaM-binding site of the IQ domain, but the missing PH1- and coiled-coil domains may also change the subcellular localization of this version of Ras- GRF1. These domains ... (F340) increases, whereas the fluorescence intensity at 380 nm (F380) decreases upon the binding of Ca 2+ . The change in the ratio of F340 ⁄ F380 determined the...
Ngày tải lên: 19/02/2014, 18:20
Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... hypothetical. Although most cystatins inhi- biting cathepsin L also inhibit cathepsin S, an inhibitory activity of cystatin F against cathepsin S has not yet been reported. The expression of the ... of the cystatin F gene and promoter. The structures of the human cystatin F (CST7)andcystatinC(CST3) genes are illustrated schematically at the top. In the upstream reg...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of flavonol synthase from Citrus unshiu pptx
... substitution of glycine residues by alanine or proline and the exchange of proline by glycine. Glycine residues are frequently found at the C-terminal ends of a helices providing the conforma- tional ... functions in many plants beyond the scope of pigmentation and ultraviolet screening, e.g. in reproduction [1], in the defense against microbial pathogens and ins...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... Consequently, the molecular studies of the interaction of SAgs with their s pecifics ligands will not only advance understanding of the physiological mechanisms of these molecules, but may lead to the development ... been shown to b e the TCR binding site in other SAgs. Point mutation of Cys26 to Ser prevented dimer formation and allowed TCR interaction studies. Initi...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx
... protein ligand tethering the co-enzyme in a productive mode within the active site and stabi- lizes the protonated N(1) of the co-enzyme to strengthen the electron-withdrawing capacity of the co-enzyme ... function instead in subgroup Ib [18]. Arg386 of the small domain binding the a-COO ) of the substrate plays a key role in the activity of the enzyme...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt
... describing the gene structure of CB1 and i ts pattern of expression in transgenic mice will provide the information necessary to determine how mutant huntingtin alters the expression of this ... in HD mouse brain. T his supports the hypothesis that the rate of transcription of CB1 in the striatum of symptomatic R6/2 mice is similar to the rate of transc...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Cloning, expression and characterization of a family-74 xyloglucanase from Thermobifida fusca pptx
... Da), indicating that the signal peptide was being cut in different places, resulting in the addition of up to 17 extra amino acids at the N terminus of the protein. It is not clear why the Xeg74CD ... [11,22] using this signal sequence and it has a convenient NotIsiteatits C terminus, which allows cloning in- frame with alanine as the first amino acid of the mature...
Ngày tải lên: 23/03/2014, 21:20