Báo cáo toán học: " Hypothermic cardiac arrest far away from the center providing rewarming with extracorporeal circulation" potx

... against the PDB [57] database. A further search for suitable tem- plates was made with the servers SAM-T02 [58,59] and FUGUE [60] and gave the same results, therefore confirm- ing the correctness of the ... reference, the position of the sugar and of the metal in the crystallo- graphic template 1YOE. The two ligands were modelled in each binding site of SsCU-NH, and then...

Ngày tải lên: 18/02/2014, 17:20

... We counted the number of tar- get terms in the following five cases. The right half (Evaluation II) in Table 2 shows the result. S: the target term was collected by the system. F: the target term ... explanation of the term. 4. There are several technical terms that are re- lated to the term. We have implemented the checking program of the first two conditions in the sys...

Ngày tải lên: 20/02/2014, 16:20

... calculated from the obtained measurements, the background fluorescence was subtracted, and the resulting value was compared with that of the AtxC sample. The influence of high ionic strength on the enzy- matic ... respectively. This PLA 2 clearly differs from the one from A. pallida. Here we report on the isolation, cloning and charac- terization of a novel PLA 2 from...

Ngày tải lên: 06/03/2014, 11:20

... beads, with the aim of both perturb- ing the MS spectrum and capturing the MCP in the body extract. The control spectra, as well as the ‘per- turbed’ one (by rPCI addition, followed by removal of the ... in the 17–37 kDa range are the most prominent. To facilitate identification, we repeated the 2D-PAGE with three different initial samples from the body, after passi...

Ngày tải lên: 07/03/2014, 02:20

... [1]. Conclusions The stability of the b-glucosidase from the hyperther- mophile P. furiosus was studied as a function of tem- perature, pressure and pH. As well as the expected high thermostability, the enzyme ... addi- tion, the band at  1.0 GPa in Fig. 5C does not resemble the broad, featureless band typical of an unfolded protein. Taken together, these observations suggest...

Ngày tải lên: 07/03/2014, 03:20

... 10 7 PMNsÆmL )1 were pre-incubated, with or without the selected peptides derived from PKI55, at 37 °C for 10 min and then stimulated with 10 nm fMLP-OMe for 2 min. The reactions were halted by the addition of ice-cold ... pre-incubated with the selected peptides derived from PKI55 for 10 min at 37 °C. The cells were then incubated with 5 lgÆmL )1 cytochalasin B for 5 mi...

Ngày tải lên: 07/03/2014, 05:20

... vesicles fuse with the ER to deliver their retrieved load. The dynamic restructuring of the ER network is enabled by the branching of existing tubules and the fusion of tubules with each other [182]. ... retain it in the ER, and (b) the protein needs to be recognized by the export machin- ery of the ER. The retention of folding intermediates can be the consequence of...

Ngày tải lên: 07/03/2014, 06:20

... over- night, with a radioactive probe made from the PCR fragment amplified with the primers used in the cloning of MCA0715. The arrowhead indicates the 540 bp transcript, corresponding to the predicted ... fall into the most favoured regions of the Ramachandran plot, 9.4% in the allowed regions and only one residue (Ala100) into the disallowed region. These results ar...

Ngày tải lên: 07/03/2014, 17:20

... chro- matography, and the recovery of the t R  34 min species decreased with a biphasic time-course with t 0.5 -values of 1.9 and 6.2 days. The fastest rate is compatible with the rate determined for the sequence-controlled ... carbonyl of Asn by the nitrogen of the peptide group linking the Asn to the following residue [4–6] 2 . As hydrolysis of this intermediate may...

Ngày tải lên: 08/03/2014, 08:20

... Louis, MO, USA) in the same buffer. The column was washed with the buffer without divalent cations until the A 280 of the eluant was minimal. The glycosylated proteins were eluted with 10 m M Tris ... as GI:AY034877. The start and the stop codons are shown in bold. The coding region is shown in upper case and the UTR is shown in lower case. The sequence of last 33 nu...

Ngày tải lên: 08/03/2014, 10:20