báo cáo hóa học:" Regulation of microRNA biosynthesis and expression in 2102Ep embryonal carcinoma stem cells is mirrored in ovarian serous adenocarcinoma patients" pdf

... unaltered in EC cells. This indicates the complex- ity of miRNA biosynthesis regulatory mechanisms in EC stem cells and tumours. This mechanism is clearly linked to higher grade malignancy and its ... differentiation of NTera2 cells. In contrast, the level of differential expression of each gene is within 1.0 fold in both undifferentiated and differentiat...

Ngày tải lên: 20/06/2014, 07:20

... phenotype is indicative of over- expression of inositol-1-phosphate synthase owing to misregulation of the INO1 gene [1,25]. The S3 strain (sec14D pct1D), containing an empty cloning vector, dis- plays ... was able to restore wild-type regulation of t he INO1 gene, derepression of the gene in medium lacking inositol, and repression in medium containing inositol. N one o...

Ngày tải lên: 30/03/2014, 15:20

... quantification of Utrophin and Tubulin (used as housekeeping protein) in Figure 1 QRT-PCR quantification of myo-miRs and miR-133 and miR-107 in biopsies from vastus lateralis of 7 DM1 patients ... expression of pro- teins important in DM1 disease pathogenesis and pro- gression. The main goal of this study is to test the hypothesis that myo-miRs expression is a...

Ngày tải lên: 18/06/2014, 16:20

... sequence involved in binding to the WW domain, a sequence of 38 to 40 amino acids containing two widely spaced tryp- tophan residues, which are involved in protein-protein interaction. In fact, ... shown to inhibit the budding of VLPs or virions [9,10,12,22,23,31,32]. In this study, to investigate the mechanism of HTLV-1 budding in detail, we analyzed HTLV-1 budding using DN f...

Ngày tải lên: 20/06/2014, 01:20

... c-Myb expression of FeLV LTRs containing c-Myb binding site mutations. (A). Diagram of the 21-bp triplication as contained in the FeLV-945 LTR, indicat- ing the sequence of c-Myb binding sites ... essential amino acids and 50 µg/ml gentamicin reagent solution (Invitrogen, Carlsbad, CA,). 3201 is an FeLV-negative thymic lymphoma cell line of feline origin [32] and was maint...

Ngày tải lên: 20/06/2014, 04:20

... promoter fragment of the human Cyr61/CCN1 gene is functional and induces a reporter gene activity in S1P-treated cells. Using a combination of cis-element mutagenesis and expression of dominant negative inhibitors ... CRE-binding protein; CBP, CREB-binding protein; B-Zip, basic leucine zipper; PKA, protein kinase A; MSK, mitogen- and stress-activated kinase; SAPK, stress- a...

Ngày tải lên: 08/03/2014, 08:20

... miR-375 and the 3¢-UTR of myotrophin mRNA was shown to repress myotrophin translation and result in the inhibition of insulin secretion. In addition to its role in exocytosis control, myotrophin is ... [65,66]. These findings represent another interesting aspect of the action of myotrophin in cells and may explain the mechanism by which miR-375 also mediates insulin e...

Ngày tải lên: 29/03/2014, 22:21

... advances in the methods for selecting and manip- ulating T cells there is increasing interest in the adop- tive transfer of bioactive T cells as a treatment for infections and cancer. This approach ... in vivo without provoking antigen induced cell death. This method also may be advanta- geous in maintaining CCR7 expression on expanding CD8 cells in settings where ce...

Ngày tải lên: 18/06/2014, 16:20

... potential of incretin targeted therapies. Curr Med Res Opin 2007, 23:1493-1507. 24. Strowig SM, Raskin P: Combination therapy using metformin or thiazolidinediones and insulin in the treatment of diabetes mellitus. ... A1C and minimize the risk of cardiovascular events. Augmentation of anti-hyperglycemic therapy in metformin-failed patients using sulfonylurea or TZD is often...

Ngày tải lên: 18/06/2014, 19:20

... psychological and physical wellbeing of the caregiver and of his/her family. Caring is held to be very demanding and emotionally involving. The main complaint of caregivers is the lack of support from ... of care. In fact, 39% of the AD patients have to be constantly supervised and 42% need to be helped also when washing and dressing, and for eating, etc., being...

Ngày tải lên: 18/06/2014, 19:20