Báo cáo hóa học: " Analysis of vaccinia virus temperature-sensitive I7L mutants reveals two potential functional domains Megan J Moerdyk1, Chelsea M Byrd2 and Dennis E " pot

... through the plasma membrane spreading outside of the cells, they are consid- ered extracellular enveloped virus (EEV). Despite intensive study of VV morphogenesis, the mecha- nism required for the transformation ... was added and removed. After the removal of rifampicin, new infection media with and without the drug was replaced. Infected cell pellets were re-suspended in PBS, sub...

Ngày tải lên: 18/06/2014, 18:20

... time of the peak accelerations of sled and head were measured. Also, the time relations of the onset and peak of the EMG were measured and analyzed. The time to onset was deter- mined when the ... Values in parentheses represent one standard deviation. Table 2: Mean Time (msec) at Which Peak Electromyogram Occurred After the Firing of the Solenoid of the Pneumatic Piston Mus...

Ngày tải lên: 19/06/2014, 10:20

... severity and posture positions were randomly varied between the 4 levels of acceleration. Each subject effectively underwent 4 levels of accelerative impacts under two conditions of trunk flex- ion, ... the effect that applied acceleration has on the left and right ster- nocleidomastoid muscles for the variables of peak electromyogram (EMG) (µV), normalized EMG (percentage of...

Ngày tải lên: 19/06/2014, 10:20

... freedom were adequate (see Table 3). Also, CFI and TLI values were adequate, whereas RMSEA values were less satisfactory. The performance measure WRMR, most important performance measure for ... and parent (see Table 1 for the contents of the items selected for each domain separately). If required, items were rephrased to meet style criteria: unambiguous, concise, easily understandable...

Ngày tải lên: 18/06/2014, 19:20

... form requires that sample ions be immersed in a dense population of near-ther- mal electrons. This requirement makes it technically challenging to implement ECD on the instruments used most commonly ... c¢ and z¢ Æ define sequences at the N-terminus and C-terminus of the protein, respectively. These sequences match to the 50S ribo- somal protein, L34. Lines above and below the protei...

Ngày tải lên: 18/02/2014, 16:20

... reduced-serum medium (Invitrogen, Carlsbad, NM, USA) twice to remove phenol red dye; then the medium was replaced by Opti- MEM I. Immediately thereafter, FCS measurements were carried out. The obtained ... of the nuage might be related to the protection of the genome via repression of the selfish genetic elements in the female germ line [3]. The nuage is known to be an electron-dense str...

Ngày tải lên: 18/02/2014, 16:20

... cells expressing httEx1-polyQ-EGFP poly- peptides, we have analyzed whether the phenomenon correlated with increased levels of Fe(II). The intra- cellular level of Fe(II) was determined (see Experi- mental ... described in the previous figures, with 2 m M N-acetyl-L-cysteine (NAC) is presented. The data from three inde- pendent experiments were used to performed statistical analysis (s...

Ngày tải lên: 19/02/2014, 06:20

... made by the designer of the lexicon. Same experiments :,a ~e been made to include a weighting mechanism, which should depend both on the syntactic context and on the semantic knowledge (Lesmo ... left to the unification mechanism of P~K)LOG, the design of the grammar is rendered s(~newhat more complex. % ~e fragment of grammar reported in (Huang, 1984) gives the i~pression...

Ngày tải lên: 21/02/2014, 20:20

... were excited at 320 nm. Measurements were performed three times. (D, E) Gel mobility shift assay in the absence of Mg 2+ . The same samples used in (A) were incubated in T buffer with 10 mM EDTA ... examine whether any Trp residues of Mhr1 are close to the DNA molecule in the Mhr1–ssDNA complex, we measured the energy transfer from the Trp residues to the fluorescent nucle- obase ethenoad...

Ngày tải lên: 06/03/2014, 09:22

... the presence and absence of 1 mm methionine the levels of LSM8–GFP mRNA expressed from P MET25 were, respectively, 3.5 and 15.5 times that of natively expressed LSM8 mRNA (Fig. S2). The level of ... Despite the differences between these models, both show a structured Lsm1p C-terminus, whereas most of the N-terminal extension of Lsm1p is predicted to be unstructured. Based on a...

Ngày tải lên: 07/03/2014, 02:20