báo cáo hóa học:" Vascular endothelial growth factor regulates osteoblast survival – evidence for an autocrine feedback mechanism" pptx

Ngày tải lên: 19/06/2014, 22:20

... Prabhakar † , Chandrika Abburi, Neel K Sharma and Akshay Anand * Abstract Background: We have earlier shown that protein levels of vascular endothelial growth factor- A (VEGF-A) and chemokine ligand-2 (CCL2) ... FM, Kanz L, Möhle R: Expression of vascular endothelial growth factor (VEGF) and its receptors in human neuroblastoma. Eur J Cancer 1999, 35:445-449. 13. Ebihara N, Yam...

Ngày tải lên: 19/06/2014, 22:20

... and human melanoma cells given recombinant VEGF increased their adhesion and proliferation via a cele- coxib-inhibitable mechanism. On the other hand, several human melanoma cell lines [34] and ... S, Sanchez-Madrid F, Parmiani G, Mantovani A, Anichini A, Dejana E: Heterogeneity in human melanoma cell adhesion to cytokine activated endothelial cells correlates with VLA-4 expression. Cancer...

Ngày tải lên: 18/06/2014, 22:20

... was performed 48 h after implanting a cannula, and tissue samples were taken 1, 3 and 7 days after the operation. Control rats were also anesthetized an d underwent operation to implant a cannula. Zhao ... (Hayward, CA). Data were normalized, and expressed as mean ± SD from 5 ani- mals, each performed in triplicate. TaqMan reverse transcription (RT)-PCR for miRNA quantification Total RNA...

Ngày tải lên: 19/06/2014, 22:20

... were quantified in the frontal cortex. Assays designed to probe for expression of IDO1’s full-size transcript (probe spanning exons 1 and 2) and one that quantified all transcripts (probe spanning ... a variant form of insulin-like growth factor I. Proc Natl Acad Sci U S A 1986, 83:4904-4907. 27. Guan J, Gluckman PD: IGF-1 derived small neuropeptides and analogues: a novel strategy...

Ngày tải lên: 19/06/2014, 22:20

... reactivase (DD-R) and the enzymeÆreactivase complex(es) (DDÆDD-R) are indicated on the tops of chromatograms. A B C 66 K– 66 K– 45 K– 36 K– 29 K– 24 K– 20 K– 14 K– Fig. 5. Analysis of the enzymeÆreactivase ... ⁄ MgCl 2 . (B, C) Bands i–vi in (A) were excised and subjected to SDS ⁄ PAGE on 6% (B) and 14% (C) gels. Lanes i–vi correspond to bands i–vi in (A). Lane D: purified enzyme. Lane R: puri...

Ngày tải lên: 15/02/2014, 01:20

... M: Pre-malignant and malignant lymphoproliferations in an HCV-infected type II mixed cryoglobulinemic patient are sequential phases of an antigen-driven pathological process. Int J Cancer 2000, ... CBA Analysis Software. Statistical analyses Intergroup comparisons were performed with the Mann-Whitney U test (for univariate nonparametric group analysis). All p-values were two-tailed and ....

Ngày tải lên: 18/06/2014, 16:20

... (antisense) were used, and the amplified products were normalized by loading an equal amount of extracted RNA for each sample. For the screening of Neu2 transfect- ants, PCR analysis was performed using ... blots. Insulin-like growth factor 1 signaling A. Fanzani et al. 3718 FEBS Journal 273 (2006) 370 9–3 721 ª 2006 The Authors Journal compilation ª 2006 FEBS Insulin-like growth...

Ngày tải lên: 19/02/2014, 06:20

... towards recombinant Rheb [21,27]. Huang and Manning [21] have indicated that phosphorylation status of TSC2 on S939 and T1462 was significantly changed with serum starvation or growth factor stimulation, ... (recombinant analog) and protease inhibitor cocktail for mammalian tissues (P8340) were obtained from Sigma-Aldrich (St Louis, MO, USA). Wortmannin and rapamycin were obtained from C...

Ngày tải lên: 15/03/2014, 11:20

... Hirakawa T, Nakano H: Expression of angiogenesis factors in monolayer culture, multicellular spheroid and in vivo transplanted tumor by human ovarian cancer cell lines. Cancer Lett 2003, 196:229-37. 22. ... Proliferation of cancer cells and recruitment of angiogenic endothelial cells and myofibroblasts were studied in subcutaneous tumors and hepatic metastases generated by subcutane- ous an...

Ngày tải lên: 18/06/2014, 15:20