... addi-
tion to the loss of cell–cell adhesion, involves degrada-
tion of the extracellular matrix and release of cells from
the constraints of cell–cell and cell–matrix interaction.
MMPs and urokinase-type ... increased expression of uPA is
directly related to higher tumor growth and metastasis
[1]. Several analyses have already made it clear that the
expression of...
... inhibition of NF-jB, either by antisense
oligonucleotide or by overexpression of IjBa, attenu-
ated both TNFa- and H
2
O
2
-induced PAI-1 expression.
TNFa was reported to induce both superoxide anion
and ... Dependence of PAI-1 expression
upon NF-jB activation. (A, B) Inhibition of
PAI-1 antigen expression produced by incu-
bation of TNFa-stimulated endothelial cells...
... gene expression was
quantified using relative quantification (RQ) units, as
described in Materials and Methods. Not all animals had
induction of gene expression of all of the above genes and
there ... PD study of Ab-01 and Ab-02 in cynomolgus mon-
keys (RQ
cutoff
) was defined as 2.3 using the formula: log
[RQ
cutoff
] = mean of the log-transformed RQ values -
standard d...
... quantification of
Utrophin and Tubulin (used as housekeeping protein) in
Figure 1 QRT-PCR quantification of myo-miRs and miR-133 and
miR-107 in biopsies from vastus lateralis of 7 DM1 patients ... repressor
factors of the wild type and mutant DMPK transcripts.
The binding of miR-103 and miR-107 to the 3'UTR of the
DMPK expanded mRNAs could therefore affects the s...
... confounding effect of tumor
DNA derived from monoclonal B cells on the quantifi-
cation of KRECs and TRECs, genomic DNA from
PBMC of 2 healthy donors with high and low number
of KRECs and TRECs was ... KRECs are randomly
present in about 5 0% of B cells released from the bone
marrow and TRECs in 70% of T cells leaving the thy-
mus, their quantification is considered...
... Child-OIDP and some of the asso-
ciations between the Child-OIDP and proxy measures
were not significant. While the low value of alpha is
affected by the nature of the Child-OIDP index and the
fact ... interview time and respondent burden, the use of
pictures have been used in the Thai version of Child-OIDP
[1,21] while a shorter version of the OIDP based only on
the ass...
... the
enzyme from E. coli [9], NadB from B. subtilis is a
dimer of 115 kDa in the absence of NaCl and a mono-
mer of 55 kDa in the presence of 150 mm NaCl. After
incubation with pure NadA in ratios of ... horikoshii and Sulfol-
obus tokadaii, and characterized from a biochemical
and structural point of view only from E. coli and
S. tokadaii [8–17]. It is a flavoprot...
... FEBS
Inhibition of Hsp90 function delays and impairs recovery
from heat shock
Roger F Duncan
1,2
1 Department of Molecular Pharmacology and Toxicology, University of Southern California School of Pharmacy, ... accumulation of a threshold level of Hsp70 sig-
nals the cessation of Hsp mRNA transcription and the
restoration of the normal rate of protein synthesis.
This...
... differentiation
and proliferation of VSMCs. Overexpression of KLF4
induced expression of multiple anti-inflammatory and
antithrombotic factors, whereas knockdown of KLF4
led to the enhancement of tumour ... addition, the effects of KLF4 on the
expression of SR-BI and the primary mechanism were
also investigated.
Results
HDL induces the expression of KLF4 and SR-BI...
... very likely by inhibition of cell
proliferation or induction of apoptosis.
MATERIALS AND METHODS
Materials
Restriction endonucleases and other enzymes for recombin-
ant DNA techniques and vectors ... The i dentity of the p
3
A
2
product synthesized by the
(2–5)A synthetase both in tissue and in primmorphs of
S. domuncula was verified by TLC and HPLC analysis as
tripho...