... extracted, and the targetZI3 1A gene was amplified by PCR with primers 5¢-AAATATAAAACGCTAGCGTCGACATGGCGC-3¢ and 5¢-AGCGTAAAGGATGGGGAAAG-3¢. The final ratio of targetcells was determined by counting ... 67–79.8 Takesako K, Ikai K, Haruna F, Endo M, ShimanakaK, Sono E, Nakamura T, Kato I & Yamaguchi H(1991) Aureobasidins, new antifungal antibiotics.Taxonomy, fermentation, isolation, and properties.J ... we have established a powerfulapproach to screen weak and transient protein–proteininteractions by incorporating a novel signal amplifica-tion circuit with intact Gc as an artificial signal ampli-fier...