... FEBS 2021 Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism Matthias Mack1, Ute Schniegler-Mattox2, Verena Peters3, Georg F. Hoffmann3, ... another function in cellular metabolism. Indeed, AUH had enoyl-CoA hydratase activity, which was described as an additionalintrinsic function of the protein. Its role in intermediary metabolism, ... brain has amolecular weight of 32 kDa (AUHp32) [15]. For thekinetic characterization of AUH described in the workat hand, AUH was overproduced in Escherichia coli asa maltose binding protein...