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Báo cáo sinh học: "Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication" pot

Tài liệu Báo cáo khoa học: Molecular and cellular specificity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

Tài liệu Báo cáo khoa học: Molecular and cellular specificity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

... axon terminal in the sinus gland, as a result of late and progressiveisomerization of the Phe3 of the CHH during the migration of the secretion vesicles along the axonaltract [39,41]. The immunohistochemical ... microscopystudy of the neuronal endings in the sinus gland todetermine the subcellular localization of the differentepimers. Taken together, these results allow a discus-sion of the existence of enzyme(s) ... immunohistochemical and immunocytochemicalresults obtained in the present study invalidate ourstarting hypothesis proposing that the cells coexpress-ing VIH and CHH in the lobster X organ, as observed in previous...
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Tài liệu Báo cáo khoa học: Molecular and biochemical characterization ofD-phosphoglycerate dehydrogenase fromEntamoeba histolytica A unique enteric protozoan parasite that possesses both phosphorylated and nonphosphorylated serine metabolic pathways docx

Tài liệu Báo cáo khoa học: Molecular and biochemical characterization ofD-phosphoglycerate dehydrogenase fromEntamoeba histolytica A unique enteric protozoan parasite that possesses both phosphorylated and nonphosphorylated serine metabolic pathways docx

... at the nucleotide binding domains and the regulatorydomain, indicating the importance of these domains for the tetramerization of the enzyme [18]. It was shown thatserine binding induces a conformational ... approximately 90% when two of the four serinebinding sites of the PGDH tetramer were bound to serine[19], indicating that the binding of a single serine at each of the two regulatory site interfaces is ... pathways. In addition to its role in the synthesis of L-cysteine and L-glycine and also in the formation of L-methionine by the interconversion of L-cysteine viaL-cystathionine,L-serineisamajorprecursorofphosphat-idyl-L-serine,...
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Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

... conformation to the proteinduring binding of the substrate is shown in the top-mostportion of the protein. ATP may bind to the glycine loopon the left flap portion (Fig. 8B) and AMP may bind to the AMP ... the enzyme. The three different views wereobtained by simple rotation of the same model. The flexible LIDdomain appears at top of the three figures, NMP-binding site and ATPbinding domain are displayed, ... acceptor (NMP),whereas the LID-domain prevents the hydrolysis of the Mg-bound phosphoryl donor in the active site. The relativemovement of the NMP-binding site and LID-domainCorrespondence to...
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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

... cyclase with the exception of an artificial isoform (CRFR1h2) with the insertion of 37 amino acids between the ligand bindingdomain and the first extracellular loop that was capable of producing detectable ... cAMP productionstimulated by urocortin. The artificial isoform (CRFR1h2)with the insertion of 37 amino acids between ligand bindingdomain and the first extracellular loop was able to produceTable ... 2004Molecular and functional characterization of novel CRFR1 isoformsfrom the skinAlexander Pisarchik and Andrzej SlominskiDepartment of Pathology and Laboratory Medicine, University of Tennessee...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

... proteincontaining a single ankyrin repeat that interacts withHIV-1 viral protein R (Vpr) and we designated thisprotein as Vpr-binding ankyrin repeat protein(VBARP). This interaction was further ... con-firmed that the cytoplasmic distribution of VBARP isindependent of the cycling stage of the cells.Identification of biological function( s) of VBARPusing a siRNA assayTo examine the role of endogenous ... that the interaction between VBARP and Vpr is specific. The input panel represents the amount of protein used in this assay indicating that anequal amount of protein was used in all our samplesand...
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Báo cáo khoa học: Functions and cellular localization of cysteine desulfurase and selenocysteine lyase in Trypanosoma brucei pot

Báo cáo khoa học: Functions and cellular localization of cysteine desulfurase and selenocysteine lyase in Trypanosoma brucei pot

... dilution. Induction of dou-ble-stranded RNA synthesis upon the addition of tet-racycline indeed resulted in efficient elimination of the SCL mRNA in two selected clones within 24 h of induction ... another function of thisprotein. The absence of Nfs disrupts Fe–S clusterassembly, monitored by the decrease in the activities of Fe–S cluster-containing proteins, such as the cytosolic and ... cell line was prepared by introducing into the insect (procyclic) stage of T. brucei strain 29-13 apZJMb vector containing a 415 bp fragment of the SCL gene. The criterion for the selection of...
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Báo cáo khoa học: Molecular and genetic characterization of osmosensing and signal transduction in the nematode Caenorhabditis elegans docx

Báo cáo khoa học: Molecular and genetic characterization of osmosensing and signal transduction in the nematode Caenorhabditis elegans docx

... ions in the cyto-plasm and functions as a chemical chaper-one that aids in the refolding of misfoldedproteins. Loss of function of protein homeo-stasis genes also causes accumulation of damaged ... responses.Extracellular solute and water balance in animals ismaintained by behavioral responses and by the func-tion of the kidney and kidney-like organs, and extra-renal organs, such as the insect hindgut, ... of damaged proteins and activation of gpdh-1expression. Green arrows and red lines indi-cate activation and inhibition, respectively.Osmosensing and signaling in C. elegans K. P. Choe and K. Strange5786...
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Báo cáo Y học: Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening pptx

Báo cáo Y học: Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening pptx

... detected in fruit (Table 1),indicating that the availability of some acyl donors is alimiting factor in vivo.Table 2 shows some kinetic properties of the recombinantCM-AAT1 protein for some of the ... transformed following the instructions pro-vided by the manufacturer (Invitrogen). The strain har-bouring the correct construction was incubated in selectingliquid medium according to Invitrogen ... for 20 min. The esters formed wereextracted with 250 lL pentane containing 5 lLÆL)1a-pinene as internal standard, vortexed for 1 min and 1 lL of the pentanic phase was injected into the GC...
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Báo cáo y học:

Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"

... (CLDN1-NLS), there is increased expression of claudin-1 in the nucleus, but there are still large amounts of claudin-1 in the cytoplasm as well. B) All of the claudin-1 in the nucleus can be shuttled into ... expression of claudin-1 in the cytoplasm, with some nuclear expression of claudin-1. B) Treatment of these cells with PKA inhibitor results in nuclear translocation of claudin-1 in as little as 15 minutes, ... dysplasia–Clefting) also have compromised skin integrity, and this has been shown to correlate to the lack of claudin-1 and also to the loss of p63 (15). Further, we have previously shown that increasing...
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Báo cáo khoa học: Concepts and tools to exploit the potential of bacterial inclusion bodies in protein science and biotechnology pdf

Báo cáo khoa học: Concepts and tools to exploit the potential of bacterial inclusion bodies in protein science and biotechnology pdf

... a-helices of the N-terminal 185 residues of the functional domain of the HA2 subunit of the in uenza virus hemagglutinin protein and to detectconformational heterogeneity of the protein within IBs[65]. ... pathways of protein folding are reflected in the formation of IBs and in their structure. Study-ing protein aggregates can therefore provide a firstglimpse about the occurrence of folding-limitingsteps. ... finddirect use in biotechnology. In addition, a detailedanalysis of the mode of building and of the structure of aggregates can be useful to dissect pathways and bottlenecks in the folding of specific...
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