... 100% abundance. The implications of a noncooperative metal-binding mechanism are that the partially metallated and metal-free species are stable intermediates, and thus may have a poten-tial role ... nonessential metal ionsin vivo and in vitro strongly suggests a role in metalion storage, metabolism and trafficking of Cu and Zn,as well as sequestration of Cd and Hg; however, theexact function of ... columns (Amersham Bio-sciences ⁄ GE Healthcare, Piscataway, NJ, USA), superfineG-25 Sephadex (Pharmacia ⁄ Pfizer, Oakville, ON, Canada) and a stirred ultrafiltration cell (Amicon Bioseparations ⁄Millipore,...