báo cáo hóa học: " The Locomotor Capabilities Index; validity and reliability of the Swedish version in adults with lower limb amputation" ppt
Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx
... After incubation with biotin, the cells were washed twice in PBSCM supplied with 100 mm glycine, and incubated for 20 min to quench further cross-linking. The quenching buffer was removed by washing ... dithioerythrei- tol-containing SDS sample buffer, and incubated for 30 min at 37 °C. The dithioerythreitol reduces the disulfide bridge in the cross-linker between biotin...
Ngày tải lên: 14/02/2014, 21:20
Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt
... include the MLL1 CXXC domain [22], a portion of the MLL1 TAD bound to the KIX domain of the cAMP response element-binding (CREB) binding protein (CBP) [23], a peptide from the Win motif of MLL1 bound ... WDR5. Consistent with a central role for the MLL1 Win motif in the interaction by WDR5, substitution of arginine 3765 with alanine in MLL1 abolishes the intera...
Ngày tải lên: 16/02/2014, 14:20
Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): evaluation of the biological function of growth-inhibitory factor in the injured and neurodegenerative brain pdf
... discovery of a brain-specific MT isoform, GIF, in 1991, sparked considerable interest in understanding the role of all MTs in the brain, and particularly within the injured or diseased brain. In the ... suggesting that this MT isoform has different and distinct functions within the brain. Furthermore, the discovery and continued investigation of this brain-spe-...
Ngày tải lên: 16/02/2014, 15:20
Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... simultaneously interacts with the main phosphate of the substrate [32]. The closer positioning of the N-terminus to the main protein body is essential for the activity and, together with the 87–104 ... 23 diminished the midpoint of the thermal denaturation curve (T m )by7°Candthefree energy of unfolding (DG U )by11kJÆmol )1 . These values are in very close agr...
Ngày tải lên: 19/02/2014, 12:20
Báo cáo khoa học: Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus niger docx
... by cotransformation of the uridine requiring strain [39] NW129 (cspA1 goxC17 pyrA6) with 1 lg of the plasmid pGW635 containing the pyrA gene and 20 lg of a plasmid containing the gene coding for the enzyme ... exponential phase and in the range of the CV of the NADPH determination. Discussion Obvious tendencies found by characterization of the wild-type...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo khoa học: Two separate regions essential for nuclear import of the hnRNP D nucleocytoplasmic shuttling sequence ppt
... Trn-1 binding sequence has been found in these naturally occurring proteins [12,17–19]. hnRNP D ⁄ AUF1 consists of two RNA binding domains (RBDs) and has a high content of glycine in the C-domain, ... encoding the D02 C-terminal 25 amino acids (282–306) and mutants of this sequence with increasing N- and C-terminal deletions as fusion pro- teins with the C-end of a...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: "Finding Deceptive Opinion Spam by Any Stretch of the Imagination" pptx
... questions, and other irrelevant or non- opinion text (Jindal and Liu, 2008). And while the presence of disruptive opinion spam is certainly a nuisance, the risk it poses to the user is minimal, since the ... presents the Turker with the name and website of a hotel. The HIT instructions ask the Turker to assume that they work for the hotel’s mar- keting departme...
Ngày tải lên: 07/03/2014, 22:20
Báo cáo khoa học: Mitochondrial transcription factor A overexpression and base excision repair deficiency in the inner ear of rats with D-galactose-induced aging pdf
... 5.1-fold, respectively. Increased protein level of TFAM induced by D-Gal To further understand the expression of TFAM protein in the inner ear, western blot analysis was performed. The protein levels of TFAM in the ... understand the protein expression of BER enzymes in the inner ear, we performed western blot analysis. The protein levels of Pol-c and OGG1 in...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Ligand binding promotes prion protein aggregation – role of the octapeptide repeats potx
... rPrP DKKRPK , recombinant PrP with deletion of GAG binding motif, KKRPK at the beginning of the N-terminal; rPrP 10OR , recombinant PrP with 10 octapeptide-repeats; rPrP 8OR , recombinant PrP with 8 octapeptide-repeats. 5564 ... Cu 2+ prevents the binding of these mAbs as shown by ELISA; Cu 2+ does not inhibit the binding of these mAbs to rPrP (results not shown). Discus...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo Y học: Propionate CoA-transferase from Clostridium propionicum Cloning of the gene and identi®cation of glutamate 324 at the active site pdf
... (in subunit A) and Ser68 (in subunit B ), are involved i n the formation of hydrogen bonds with the e-carboxylate of glutaryl-CoA and t hat the latter s erine is located within this subdomain. ... of 56 553 Da and a n isoelectric point of 4.9 1 was predicted for the encoded p rotein. B oth values w ere in agreement with the observed molecular mass and the e...
Ngày tải lên: 17/03/2014, 17:20