... efficacy of the viral vectors was assessed with the use of GFP reporter. In the assay, cells were plated in24-well plates with a density of 105 cells per well the daybefore infection. Then the ... hours) following addition of the viruses to the cells by immunofluorescent staining,and found that the distribution of Ad5.PTDtat inside the cells was similar to that of unmodified Ad5 vectors ... 1Division of Human Gene Therapy, Department of Medicine, University of Alabama at Birmingham, Birmingham, USA, 2Division of Human Gene Therapy, Departments of Pathology, University of Alabama...