Báo cáo sinh học: " Construction and characterization of recombinant flaviviruses bearing insertions between E and NS1 genes" docx

... viruses bearing genetic insertions between the E and NS1 genes must be stable to be useful for the development of new live attenuated vaccine viruses expressing antigens of other pathogens. The genetic ... YF 17D and 17D-Dengue 4 (YF17D/DEN4) viruses bearing the green fluorescent protein gene between E and NS1. We have characterized foreign gene expression and gen...

Ngày tải lên: 18/06/2014, 18:20

... committee chaired by the MoH Directorate of Human Resources and Administration and comprised of other government and partner stakeholders. Progress updates and the final report were then presented ... declare that they have no competing interests. Received: 23 December 2009 Accepted: 22 September 2010 Published: 22 September 2010 References 1. The world health report 2006: working...

Ngày tải lên: 18/06/2014, 17:20

... BamHI, EcoRI, HindIII and SacI), the Southern results indicated that there was some divergence between these sequences as evinced by the SpeI, SphI and XbaI digestions, since the predominate fragments ... of even one extra nucleotide at the 5' end of the negative sense vRNA abrogates the function of this molecule. Therefore, if no pol I promoter element is present in the clon...

Ngày tải lên: 18/06/2014, 18:20

... the same batch of virus. Three clones from two independent cloning events were fully sequenced and the identical mutations were found in all three clones. Thus, these three mutations were not ... first generation of recovered virus (designated as R1), which could efficiently infect permissive cells to produce a second generation of recovered virus (designated as R2). The genome o...

Ngày tải lên: 18/06/2014, 18:20

... thiocya- nates are formed in other species of the Brassicaceae under the influence of certain protein factors [10–12]. For example, epithiospecifier proteins (ESPs) have been identified in several species of ... assays per- formed under standard conditions as described in Experimental pro- cedures. Test parameter Effect on ESP Temperature Higher absolute and relative nitrile formation at...

Ngày tải lên: 23/03/2014, 11:20

... were subsequently modelled as alanine. All of these residues are located on the surface of the protein and are presumed to be disordered. Three N-acetylgluco- samine and 175 w ater m olecu les ... sites (Fig. 1). Sequence analysis Peptides sequenced from native CBH I B confirmed the identity of the T. emersonii cel7 gene/gene product; the location of these peptides in the deduced p...

Ngày tải lên: 23/03/2014, 13:20

... greater benefits in the long-term by, for example, ensuring a high level of treat- ment adherence, keeping patients on first line treatment for longer and deferring the use of more expensive sec- ond-line ... just resources) to develop positive synergies between antiretroviral therapy scale-up and health systems strengthening. It will also require the right balance between the use...

Ngày tải lên: 18/06/2014, 17:20

... be engaged to take over some elements of their own care. The objective is a streamlined, rationalized chain of care that relieves pressure on each worker involved while maintaining quality standards ... further results. Grey literature was searched online, and conference proceedings searched for abstracts. Results: We found 2960 articles, of which 84 were included in the core review....

Ngày tải lên: 18/06/2014, 17:20

... involvement is determined by the type of disease targeted, available intervention options and the endemicity level [6,9,16,19]. The constituent activities of vector control can be implemented either intermittently, ... for the same st rategy, to produce quite different effects; where there is a high levelofsocialsolidarity,communities will actively involve them selves, whereas where the...

Ngày tải lên: 18/06/2014, 17:20

... flow cytometry. The percentages indicated represent an average of at least 3 independent experiments. Errors bars corre- spond to the standard error of the mean. C. PBMC were inoculated with T1 ... lifetime." Sir Paul Nurse, Cancer Research UK Your research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately upon acc...

Ngày tải lên: 18/06/2014, 18:20