Báo cáo sinh học: " Recombination-ready Sindbis replicon expression vectors for transgene expression" pdf

... Central Page 1 of 11 (page number not for citation purposes) Virology Journal Open Access Methodology Recombination-ready Sindbis replicon expression vectors for transgene expression Brian J Geiss*, Lisa ... recombination cassettes into replicon expression plasmids make Sindbis replicons much easier SINV replicons can express epitope tagged mosquito genes for several...

Ngày tải lên: 18/06/2014, 18:20

... Central Page 1 of 11 (page number not for citation purposes) Virology Journal Open Access Methodology Recombination-ready Sindbis replicon expression vectors for transgene expression Brian J Geiss*, Lisa ... recombination-based system for manipulating expression cassettes in Sindbis virus replicons and show their utility for expressing transgenes of interest in cul-...

Ngày tải lên: 20/06/2014, 01:20

... shortages cannot afford for their existing health workers to be away from service sites for long periods for training, especially training that does not yield results. Learning for performance (LFP) ... Central Page 1 of 4 (page number not for citation purposes) Human Resources for Health Open Access Commentary Focusing on the essentials: learning for performance Catherine J Mur...

Ngày tải lên: 18/06/2014, 17:20

... rural THCs and 89 urban CHCs were selected for a questionnaire survey. From these facilities, 30 heads of the THCs and CHCs were selected for key-informant interviews. Investigators came from ... investigators. Question guidelines were used in the key-informant inter- views. Questions for the review included why some health professionals would leave for new institutions and how health...

Ngày tải lên: 18/06/2014, 17:20

... exclusive formula feeding then the public health service provides formula milk to the mother at no cost for the first six months. However, studies have shown that it is very difficult for mothers ... infectious disease mortality through formula feeding balance each other out [17,21]. Therefore, in South Africa, HIV-positive mothers are advised to choose between exclusive formula feeding a...

Ngày tải lên: 18/06/2014, 17:20

... dis- trict level information needed to track changes and understand human resources for health at the district level. Because policy for human resources in health is weak and civil service reform has stalled, ... resources in health. For political reasons the central governme nt decided to convert various forms of contract workers (mostly PTT for doctors and midwives and only local co...

Ngày tải lên: 18/06/2014, 17:20

... conditions: 50°C for 2 minutes, 95°C for 2 minute, 45 cycles of 95°C for 15 seconds 55°C for 30 seconds and 72°C for 30 seconds. Data analysis The ΔΔCT [23] method was used for initial data analysis. Four ... recommendations for oligo(dT)20 primed cDNA-synthesis. cDNA synthesis from RNA was performed using 1 μg of RNA, at 50°C. The cDNA was then treated with RNase H (Invitrogen)...

Ngày tải lên: 18/06/2014, 18:20

... clone TU116,BDBiosciences)byincubating the cells in 100 μL PBS for 10’ at 4°C. Cells were then washed with PBS, and fixed with 1% formaldehyde for 20’ at 4°C before perme abilization for intracellular staining. To permeabi- lize ... enrich for circulating tumor cells (CTCs) is that there might be CTCs with insufficient expression of the surface target marker which may be missed by t...

Ngày tải lên: 18/06/2014, 19:20

... Correspondence: ppl@stanford.edu 1 Department of Medicine, Division of Hematology, Stanford University School of Medicine, Stanford, California, USA Full list of author information is available ... (Invitrogen). Cycling of cDNA involved denaturation at 95°C for 30s , a nnealing at 50°C for 1 min and extension at 70°C for 1 min for 40 cycles using the iCycler iQ ™ (BioRad). Fluorescenc...

Ngày tải lên: 18/06/2014, 22:20

... applied for a broad range of analytical applications. A comparative study with gas chromatographic analysis will be performed to evaluate more precisely the performance of our Au-AAO sensor for ... Provisional PDF corresponds to the article as it appeared upon acceptance. Fully formatted PDF and full text (HTML) versions will be made available soon. A nanoporous interferometric micr...

Ngày tải lên: 18/06/2014, 22:20