... residues W101 and W162 areinvolved in the b inding of the UDP-galactose donor, t heresidue W315 in the binding of the N-acetylglucosamine-b-p-nitrophenol acceptor, and the domain including E264appears ... found in b1,3-GalTs and b1,3-N-acetylglucosaminyltransferases,thereby suggesting that they are more likely involved in mediating the b1,3-linkage speci®city r ather than in t hedirect binding of ... [37] and of the bovine b1,4-GalT-I [29], two DXD-containing glycosyltransferases, s howed an aromatic resi-due, which is involved in the stacking of t he uracil ring of the cosubstrate in the...