Báo cáo sinh học: " Characterization of vaccinia virus A12L protein proteolysis and its participation in virus assembly" pptx

... evaluated. Twenty five l of erythrocyte sample was added to each of a series of 2.5 ml saline solutions containing 0.0 to 0.8 g% of NaCl. After gen- tle mixing and standing for 15 min at room tempera- ture, ... loading of pravastatin into erythrocytes was directly proportional with increase of drug concentration in the incubation medium, in the 2-10 mg/ml concen- trati...

Ngày tải lên: 25/10/2012, 11:10

... gigas [5]. Interestingly, this protein, named C. gigas chitinase-like protein 1 (Cg- Clp1) was found to be involved in the control of growth and remodelling processes in a manner similar to its YKL-40 ... together chitinases and proteins devoid of catalytic activity due to the substitution of a critical amino acid in the cata- lytic centre. This latter singular class of...

Ngày tải lên: 19/02/2014, 00:20

... sequence, both in the amino terminus. TS, composed of residues T169–T186, resides in the carboxy-extension and is augmented in threonine and serine. Deletion of R had more in uence than removal of G on ... proposal, the protein pro- tects citrate synthase against heat-induced aggregation and inactivation of its enzymatic activity and shields insulin from dithiothrei...

Ngày tải lên: 30/03/2014, 20:20

... con- taining DNA spots (5 lg each) corresponding to inter- nal fragments of ADC or cruzipain genes. The latter (used as a control) is a housekeeping gene that encodes the main cysteine proteinase of ... radioactive bands obtained in the corresponding Southern blot assay (Fig. 3B) could only be explained by the integration, into the parasite genome, of ‡ 2 units of the plasmid pADC-8...

Ngày tải lên: 19/02/2014, 07:20

... composed of 33 exons coding for a protein of 165 kDa, containing a class II (purF)-type glutaminase domain and short regions for binding to FMN and iron sulfur center. GLT is composed of 20 Amino ... (ORN), citrulline (CIT) and arginine (ARG) contents in leaves of Arabidopsis plants cultured with 5 m M nitrate in air. (F) Ornithine, citrulline and arginine contents in...

Ngày tải lên: 30/03/2014, 01:20

... further investi- gations in the future. In conclusion, we found a high prevalence of B19 infection in livers from patients with fulminant hepa- titis and biliary atresia. Interestingly, all fulminant ... length of 5.4 kb with hairpin structures at each extremity. B19 encodes three major viral proteins: VP1 and NP2, the viral capsid proteins, and NS1, a nonstructural protei...

Ngày tải lên: 26/10/2012, 10:03

... methods, and interdisciplinary interest in its application as a prognostic, diagnostic, and investigative tool, an understanding of the underpinnings of P300 and N200 physiology may support its ... diagnostic and feedback tool are growing more and more promising, and as we gain more insight into the nature of the ERP, the search for clinical integration will assur...

Ngày tải lên: 02/11/2012, 11:08

... sine function was applied in both dimensions of the time domain before Fourier transformation. Integration of 1D and 2D signals was performed using 1D winnmr and 2D winnmr software (Bruker, AG, Darmstadt, ... reported. 1D and 2D data were in good agreement, although the starting intensity values and values at the minimum were different, reflecting different pools of ML inside...

Ngày tải lên: 18/02/2014, 13:20

... indicate intron 6 of 75 bp in fragments B and C, 3¢ portion of 35 bp in intron 5 in fragments C and D, and 3¢ portion of 25 bp in intron 6 in fragments F and I. The number at the right of each RT-PCR ... contain the entire coding region. Thus, use of alternative promoters in DNASE1 results in no generation of protein isoforms. The 5¢-UTR of eukaryotic mRN...

Ngày tải lên: 19/02/2014, 06:20

... using crotonyl-CoA as a substrate [15]. The gene encoding this bifunctional protein was named AUH (‘AU bind- ing homolog of enoyl-CoA hydratase’). The RNA- binding activity of the human protein ... a molecular weight of 32 kDa (AUHp32) [15]. For the kinetic characterization of AUH described in the work at hand, AUH was overproduced in Escherichia coli as a maltose binding p...

Ngày tải lên: 19/02/2014, 07:20