Báo cáo sinh học: " Virus detection and identification using random multiplex (RT)-PCR with 3''''''''-locked random primers" docx

... Access Research Virus detection and identification using random multiplex (RT)-PCR with 3'-locked random primers Amy L Clem, Jonathan Sims, Sucheta Telang, John W Eaton and Jason Chesney* Address: ... method for rapid virus detection and identification based on random multiplex (RT)-PCR using 3'-locked random primers to avoid primer-dimer...

Ngày tải lên: 18/06/2014, 18:20

... trans- fected with plasmids encoding NP or NP∆C1 together with a plasmid encoding T7 polymerase. Cells were fixed with 4% paraformaldehyde at 18 h post transfection and incubated with a rabbit ... intracellular inclusions, interac- tion of NP with VP35 and for the function of NP in RNA synthesis. Materials and methods Cells and cDNA transfections HeLa, HUH7 and HUH-T7 ce...

Ngày tải lên: 18/06/2014, 18:20

... were drawn before (T0, T1m) and 24 h after (T0+24, T1m+24) the first and the second vaccine administration and cells were isolated by ficoll gradient-centrifugation and labeled with specific antibodies, ... CD16 and low levels of CD14, wer e first characterized by Ziegler- Heitbrock and colleagues in 1988 [41], and their number and phenotype/function have been reported to be...

Ngày tải lên: 18/06/2014, 19:20

... identified as a receptor for the alphaviruses Sindbis virus[ 24] and Venezuelan equine encephalitis virus (VEEV)[25] and the flavivirus tick-borne encephali- tis virus (TBEV)[26]. In our study, 2D ... [4-11]. Heparan sulfates[12] and the C-type lectins DC-SIGN and L-SIGN have been shown to mediate infection by dengue viruses[13] and most recently, studies using a standard vi...

Ngày tải lên: 19/06/2014, 08:20

... stand- ard. 2077 annotations remain wrong independently of the changes in the gold standard. 1131 of the re- lations become wrong with the changed gold standard, whereas they were correct with ... Automatic Detection of Errors We propose a different approach. We take the Eng- lish dependency treebank and train models with two different state of the art parsers: the graph- based M...

Ngày tải lên: 07/03/2014, 22:20

... helpful discussions with each other, Diane Horton, and Hector Levesque, and finan- cial support from IBM, the Natural Sciences and Engineer- ing Research Council of Canada, and the University ... variable, and P is the new predication on the variable. This reflects observations of Moore (1981) and others that determiners rarely have a direct correlation with the existent...

Ngày tải lên: 31/03/2014, 17:20

... in Maputo, with the aim of identifying their social and geo- graphical origins and their expectations and difficulties regarding their education and professional future. Methods A piloted, standardized ... satisfied or partially satisfied with the burden of lecturing and learning hours demanded by the medical school. Twenty-six percent were unhappy or partially unhappy with it...

Ngày tải lên: 18/06/2014, 17:20

... purposes) sonnel policy and procedures, and produced and distrib- uted a new personnel manual to management and supervisory staff at the clinics. Personnel files were com- pleted and job descriptions ... develop a monitoring and evaluation plan using indicators that would track HR management and perform- ance components. MSH staff and the FLEP Human Resource Administrator me...

Ngày tải lên: 18/06/2014, 17:20

... acquainted with Malian laws and regulations related to health centre functioning, and with practical skills and tools related to financial management, human resource management, and drug management ... poor career perspectives and training opportunities, unsatisfactory access to sup- plies and support mechanisms, and disappointing human interactions with colleagues and m...

Ngày tải lên: 18/06/2014, 17:20

... capacity to diagnose and handle patients adequately. Thus, in Sen- egal in 2002, maternal morbidity was significantly better diagnosed and treated by doctors and midwives than by nurses and traditional ... one hand, ethical and logistical constraints, and on the other, the extent to which staff performance depends on material and organizational resources [47,48], existing theore...

Ngày tải lên: 18/06/2014, 17:20