Báo cáo sinh học: " Virus detection and identification using random multiplex (RT)-PCR with 3''''''''-locked random primers" docx
Báo cáo sinh học: " Virus detection and identification using random multiplex (RT)-PCR with 3''''-locked random primers" docx
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Research
Virus detection and identification using random multiplex
(RT)-PCR with 3'-locked random primers
Amy L Clem, Jonathan Sims, Sucheta Telang, John W Eaton and
Jason Chesney*
Address: ... method for rapid virus detection
and identification based on random multiplex (RT)-PCR
using 3'-locked random primers to avoid primer-dimer...
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... trans-
fected with plasmids encoding NP or NP∆C1 together with a
plasmid encoding T7 polymerase. Cells were fixed with 4%
paraformaldehyde at 18 h post transfection and incubated
with a rabbit ... intracellular inclusions, interac-
tion of NP with VP35 and for the function of NP in RNA
synthesis.
Materials and methods
Cells and cDNA transfections
HeLa, HUH7 and HUH-T7 ce...
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... were
drawn before (T0, T1m) and 24 h after (T0+24, T1m+24) the first and the second vaccine administration and cells were isolated by ficoll
gradient-centrifugation and labeled with specific antibodies, ... CD16 and low
levels of CD14, wer e first characterized by Ziegler-
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... identified as a receptor for the alphaviruses
Sindbis virus[ 24] and Venezuelan equine encephalitis
virus (VEEV)[25] and the flavivirus tick-borne encephali-
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In our study, 2D ... [4-11]. Heparan sulfates[12] and the
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mediate infection by dengue viruses[13] and most
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... stand-
ard. 2077 annotations remain wrong independently
of the changes in the gold standard. 1131 of the re-
lations become wrong with the changed gold
standard, whereas they were correct with ... Automatic Detection of Errors
We propose a different approach. We take the Eng-
lish dependency treebank and train models with
two different state of the art parsers: the graph-
based M...
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... helpful discussions with
each other, Diane Horton, and Hector Levesque, and finan-
cial support from IBM, the Natural Sciences and Engineer-
ing Research Council of Canada, and the University ... variable, and P is the new predication on
the variable. This reflects observations of Moore
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direct correlation with the existent...
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Methods
A piloted, standardized ... satisfied or partially
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me...
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... acquainted with Malian laws and regulations related to health centre functioning,
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plies and support mechanisms, and disappointing human
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báo cáo sinh học:" Human resources and the quality of emergency obstetric care in developing countries: a systematic review of the literature" potx
... capacity to
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