Báo cáo sinh học: " Usefulness of Herpes Consensus PCR methodology to routine diagnostic testing for herpesviruses infections in clinical specimens" docx

... Consensus PCR assay can be useful to facilitate the routine diagnosis of herpesviruses infections, within a single assay, single clinical sample, thereby allowing earlier application of specific ... experience of a single clinical microbiology laboratory has been reported since the commercial availability of the improved Herpes Consensus PCR assay in Greece. The...

Ngày tải lên: 18/06/2014, 18:20

... Consensus PCR assay can be useful to facilitate the routine diagnosis of herpesviruses infections, within a single assay, single clinical sample, thereby allowing earlier application of specific ... experience of a single clinical microbiology laboratory has been reported since the commercial availability of the improved Herpes Consensus PCR assay in Greece. The...

Ngày tải lên: 20/06/2014, 01:20

... empowered to abstain from sex or nego- tiate safe sexual practices due to fear of losing financial support [22]. 6. Staff training and welfare Aim To empower staff to fulfil their evolving roles in ... institution in Ghana, in addition to having it reviewed by independent experts in higher education. The principles outlined in the handbook help to provide a regulatory...

Ngày tải lên: 18/06/2014, 17:20

... vaccination are a critical issue in the event of mass vaccination, understand- ing the basis of MVA attenuation may lead to the develop- ment of better vaccine vectors. In this study, a number of differences ... cell lines, where virus infec- tion has been described to be productive [9] and abortive [15] in different cell lines. Our results point to a significant preferenc...

Ngày tải lên: 18/06/2014, 22:20

... to make 100 µl of viral suspension containing 64 HAU to 0.001 HAU of JCV. DNA was extracted using a QIAprep Miniprep Spin Kit and eluted in 50 µl of the elution buffer. Two µl of the 1:10 diluted ... develop this disease [6,7]. Factors influencing PML pathogenesis, including modes of JCV transmission, its dissemination from site(s) of initial infection, the mechanism(s) o...

Ngày tải lên: 19/06/2014, 08:20

... type infected. Thus it is not possible to identify a single housekeeping gene for use in QPCR studies of viral infections. Nevertheless it is of benefit for researchers to be able to determine ... RNase H (Invitrogen) and diluted 1:100 before use for QPCR. Quantitative PCR In order to measure housekeeping gene expression, cDNA was subject to QPCR using the platinum...

Ngày tải lên: 18/06/2014, 18:20

... immu- noglobulin hypervariable domains, intra-chain disul- phide bonds can, to varying degrees among different antibodies, influence conformation of the antigen binding site [31]. Thus, failure to form intra-chain disulphide ... adapted to promote formation of intra-chain disulphide bonds in vitro, using protein extracted from bacterial inclusion bodies. After isolation of inclu...

Ngày tải lên: 18/06/2014, 22:20

Ngày tải lên: 03/11/2012, 11:01

... properties of soluble HSF2 proteins, by measuring their remaining capacity to bind their target sequences after exposure to denaturating temperatures. The remaining ability of the factors to bind a consensus ... lgÆmL )1 pepst- atin and aprotinin), proteins specifically bound to the resin were eluted by steps of increasing NaCl concentration (0.3 to 2 M ). Fractions were anal...

Ngày tải lên: 24/03/2014, 00:21

... training were instrumental. The FWAs almost universally maintained the recommended protocol for administering injectables even after the withdrawal of the interventions. The high coverage of routine ... survey. Results Observation of routine activities of FWAs Data of 1995 and 1996 showed a consistent pattern of adherence to the recommended protocol for administra- tion...

Ngày tải lên: 18/06/2014, 17:20