báo cáo hóa học:" Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson''''''''s disease" doc

... Access Research Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson's ... pro- tein bands. The protein DAT could not be detected in RPE cells. (B) The cDNA of DDC but not DAT was detected by PCR from the total cDNA of...

Ngày tải lên: 18/06/2014, 15:20

... factor receptor type β (PDGFR-β) [10], and expanded these cells for induction to MC in the presence of PDGF-BB and 1% FCS. At passage 5, all of the expanded cells effectively differentiated into ... mononuclear cell layer was harvested in the interface and resuspended in PBS (3 × 10 6 cells/ 50 ul) for the trans- plantation. Immunohistochemical examination of cultur...

Ngày tải lên: 18/06/2014, 15:20

... Trypsin (0.05%) and EDTA (Invitrogen), and passing through 22 G needle and 40 μm cell strainer. Single cells were resuspended in Stemline II medium at a concentration of 2–5 × 10 6 cells/ ml and ... understand the immunological features of ES cell derived progenitors. In this study, we examined the expression profile of HLA molecules on the surface of human ES cell...

Ngày tải lên: 18/06/2014, 15:20

... mono- cyte-like cells were described as having ability to differen- tiate into endothelial cells in vitro and in vivo based on expression of CD31, eNOS, and E-selectin [31]. These studies were expanded into ... in response to long-term demand may be occurring. If the reparatory demands of the injury indeed lead to depletion of EPC progenitors, then administration of...

Ngày tải lên: 18/06/2014, 15:20

... expression of pro-apop- totic and anti-apoptotic proteins of Bcl-2 [ 31,32] and Caspases [33,34] families to evaluate intrinsic and extrinsic path ways of apoptosis in normal ileal pouches. Even in such ... in vivo inhibition of insulin receptor substrate-1 expression leads to insulin resistence, hyperinsulinemia, and increased adiposity. Endocrinology 2005, 146:1428...

Ngày tải lên: 18/06/2014, 16:20

... lead- ing to the differentiation of monocytes into cells with properties of Langerhans cells [15 -17]. Furthermore, DC can also be induced in the presence of IFN-b and IL-3 [18,19]. The induction of ... Presentation of antigens by iDC leads to T cell anergy, deletion of T cells or the induc- tion of IL-10-secreting T regulatory cells [8,9]. Following antigen up...

Ngày tải lên: 18/06/2014, 16:20

... of COX-2 protein was also analyzed in cells incubated with the inflammatory cytokine interleukin-1 β (IL-1β,10 ng/ml). (d) Expression of COX-2 mRNA, analyzed by semiquantitative RT-PCR in cells ... observations showing that, in certain cell lines, RAR and RXR inhibit COX-2 promoter activity [30,31]. Finally, our own studies in renal mesangial cells indicate that RARs a...

Ngày tải lên: 19/06/2014, 22:20

... cytometry and micro scopy [15,21], suggesting distinct regulation of PD- L1 and PD-L2 by endothelial cells in different species and in different organs. Massive infiltration of immune cells into ... immune cells, including T cells [12]. The interaction between PD-1 and its ligands (PD-L1 or PD-L2) suppresses T cell responses including prolifera- tion, cytokine product...

Ngày tải lên: 19/06/2014, 22:20

... cells, the relative contribution of each cell type could not be determined. CD8+ T cells and NK cells inhibit virus replication in vitro through a variety of mechanisms, including cytolytic and ... Department of Medicine, University of California, Irvine School of Medicine, Irvine, California, USA and 3 Department of Pathology, Microbiology and Immunology, Sch...

Ngày tải lên: 20/06/2014, 01:20

... interaction of acute ethanol on Jak-Stat and MAPK pathways in Huh7 cells, HCV replicon cells lines, and primary human hepatocytes. Materials and methods Cells and chemicals Human hepatoma Huh7 cells ... dependent fashion, and this coincided with a dose-dependent increase in total Stat1 protein, a known ISG. When cells were treated with a single dose of 100 mM ethano...

Ngày tải lên: 20/06/2014, 04:20