... the employed protein dissolved in an appropriate solvent (35,36). Exemplarily, lyophilized Sfl is dissolved in HFIP and cast on a surface like polyethylene (PE). After evaporation of HFIP, the ... repetitive sequences 15 From: Methods in Molecular Biology, vol. 474: Nanostructure Design: Methods and Protocols Edited by: E. Gazit and R. Nussinov © Humana Press, Totow...
Ngày tải lên: 04/06/2014, 15:06
... motility and migration and simultaneously track specific proteins tagged with complemen- tary fluorescent probes. 3.1.3. In Vivo Applications Several reports have appeared utilizing quantum dots in vivo. ... new opportunities in multiplexed assay development and greatly facilitate the study of cell/cell interactions and other complex phenotypes in mixed cell populations. 4. Fu...
Ngày tải lên: 04/06/2014, 15:14
basic cell culture protocols methods in molecular biology - cheryl d. helgason, cindy l. miller
... AM19 20 Uphoff and Drexler • Mycoplasma-positive samples show a band at 502–520 bp, depending on the mycoplasma species. In the case of Acholeplasma laidlawii contamination and applying the DSMZ internal ... deoxyadenosine triphosphate (dATP), deoxycytidine triphosphate (dCTP), deoxyguanosine triphosphate (dGTP), and deoxythymidine triphosphate (dTTP) (Peqlab, Erlangen, Germany) in...
Ngày tải lên: 08/04/2014, 12:50
cell cycle control mechanisms and protocols methods in molecular biology - tim humphrey, gavin brooks
... conserved checkpoint complexes: a complex comprising Mec1 and Ddc2 in budding yeast (132) (Rad3 and Rad26 in fission yeast [132,133]); the checkpoint loading complex, comprising Rad24 and replication ... the APC in the M/G 1 phase, and is phosphorylated in a checkpoint-dependent manner (112). In vitro assays have shown that DDK phosphorylates Mcm2-4, Mcm6, and Cdc45 (113,...
Ngày tải lên: 08/04/2014, 12:50
advances in polymer science, v.220. self-assembled nanomaterials ii. nanotubes, 2008, p.199
... However, further increase in the mole fraction of 4b results in a decrease of the CD intensity. Thus, only the nanotubes are CD active. 3 Porphyrins and Phthalocyanines Since porphyrin and phthalocyanine ... and negatively charged porphyrin derivatives [21]. When porphyrins 5 (Fig. 5a) and 6a (Fig. 5b), appended with sulfonate and 4- pyridyl groups, respectively, are allowed to...
Ngày tải lên: 04/06/2014, 15:04
molecular methods in developmental biology
... Microinjection 99 99 From: Methods in Molecular Biology, Vol. 127: Molecular Methods in Developmental Biology: Xenopus and Zebrafish Edited by: M. Guille © Humana Press Inc., Totowa, NJ 9 Preparation ... make specific null mutants in Xenopus laevis has recently been overcome, in part, by the overexpression of ingenious vari- ants of the endogenous proteins under study. T...
Ngày tải lên: 11/04/2014, 09:52
data analysis in molecular biology and evolution - xuhua xia
... of the rapidly expanding interdisciplinary science. In short, the material is developed in the spirit of the student-centered learning which is now gaining acceptance and popularity in universities ... again for holding the book with caring hands. May the book and the program be useful to you! Preface People learn by observing things around them. When the telescope and the micros...
Ngày tải lên: 08/04/2014, 12:50