dna polymer
Tài liệu Báo cáo khoa học: Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase d and S6K1 and regulating cell growth docx
... hybridizations with cDNA probes The human multiple tissue expression array from Clontech (#7775-1) was hybridized with two radiolabeled human cDNA probes. The cloned ER cDNA was labeled using ... molecular partners of the p50 subunit of DNA polymerase d [23]. No further data on this interaction and the possible role of PDIP46 in processes in which this polymerase takes part have been report...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Dissociation of DNA polymerase a-primase complex during meiosis in Coprinus cinereus pptx
... Purification step of C. cinereus DNA polymerase a. One unit (1 U) of DNA polymerase was defined as the amount needs to catalyze the incorporation of 1 pmol of [ 3 H]-d TTP into a DNA polymer in 30 min. Protein ... should be noted that intrinsic DNA polymerase activity in fraction II has quite low processivity for DNA synthesis which is a typical feature for replicative DNA polymer...
Ngày tải lên: 20/02/2014, 11:20
Báo cáo khoa học: The N-terminal region of the bacterial DNA polymerase PolC features a pair of domains, both distantly related to domain V of the DNA polymerase III s subunit ppt
... the C-family of DNA polymerases [2]. Polymerases of the C-family fall into two major groups, DnaE and PolC, typified respectively by Escherichia coli PolIIIa and Bacillus subtilis PolC. DnaE and PolC ... PolC and DnaE is of a different nature. DnaE, much like eukaryotic DNA polymerase a, initially extends an Abbreviations OB, oligonucleotide ⁄ oligosaccharide-binding; PDB, Protein Data Ban...
Ngày tải lên: 05/03/2014, 23:20
Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
... polynucleotide kinase, E. coli DNA polymer- ase I, the Klenow fragment of E. coli DNA polymerase I lacking 3¢ to 5¢ exonuclease activity, Taq DNA polymerase and pfu DNA polymerase were from Takara ... Doxorubicin did not inhibit the exonuclease activity of T7 DNA polymer- ase, whereas for Escherichia coli DNA polymerase I, both exonuclease and polymerase activities were seen....
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khoa học: DNA polymerase e associates with the elongating form of RNA polymerase II and nascent transcripts pot
... 2006) doi:10.1111/j.1742-4658.2006.05544.x DNA polymerase e co-operates with polymerases a and d in the replicative DNA synthesis of eukaryotic cells. We describe here a specific physical interaction between DNA polymerase e and RNA polymerase ... RNA polymerase IIO and DNA poly- merase e, and immunoelectron microscopy revealed RNA polymerase IIO and DNA polymerase e in defined nuclea...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues docx
... relationship between OsPol k and other X-family DNA polymerases, a tree was drawn based on the amino acid sequences of the DNA polymerase domains Fig. 2. DNA polymerase activity of OsPol k protein. (A) ... effects of DNA polymerase inhibitors on the DNA polymerase activity of OsPol k. Strong inhibition was noted with 2,3-dideoxythymidine-5-triphosphate, a known inhibitor of mammalia...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: The noncatalytic C-terminus of AtPOLK Y-family DNA polymerase affects synthesis fidelity, mismatch extension and translesion replication ppt
... (2000) Human DNA polymerase j synthes- izes DNA with extraordinarily low fidelity. Nucleic Acids Res 28, 4147–4156. 20 Wolfle WT, Washington MT, Prakash L & Prakash S (2003) Human DNA polymerase ... AtPOLK DNA polymerase FEBS Journal 274 (2007) 3340–3350 ª 2007 The Authors Journal compilation ª 2007 FEBS 3349 of high-fidelity and error-prone DNA polymerases and ligases involved in...
Ngày tải lên: 16/03/2014, 10:20
Báo cáo khóa học: Crystal structure of the chi:psi subassembly of the Escherichia coli DNA polymerase clamp-loader complex doc
... archaebacteria] that tether the DNA polymerase to DNA [2]. The interaction between the DNA polymerase and the sliding clamp enables the active site of the polymerase to bind and release DNA rapidly during ... other DNA polymerases by its ability to rapidly polymerize tens of thousands of nucleotides without dissociating from the template. This high level of processivity is conferred...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase ppt
... and murine DNA polymerase a-primase, respectively; columns 4 and 5, JCV Tag with human and murine DNA polymerase a-pri- mase, respectively. DNA polymerase (0.5 U) of the indicated DNA polymerase ... host factor responsible for species specificity is DNA polymerase a-primase, which initiates DNA replication in all eukaryotes [19,22–27]. DNA polymerase a-primase consists of four su...
Ngày tải lên: 17/03/2014, 03:20
Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot
... acid binding in either the polymerase domain [33] or the RNase H domain [32– 36], alter the RNase H cleavage position of the RNA :DNA hybrid. In addition, a subset of polymerase domain primer grip ... catalytic activities: (a) DNA poly- merase activity, which can be both RNA and DNA dependent; and (b) RNase H activity that selectively hydrolyzes the RNA strand of the RNA :DNA hybrid for...
Ngày tải lên: 22/03/2014, 16:20