Single cell protein introduction
Single cell protein introduction
... Single cell protein introduction Single cell protein conceptSingle cell protein concept Single cell protein is a terms for a type of cell nutrient and it produced ... of single cell protein synthesis as: bacteria, yeast, filamentous fungi, algae Yeast BacteriaAlgae Filamentous Fungi Advantages and disadvantages• Advantages of single cell protein: •...
Ngày tải lên: 03/01/2013, 12:52
Báo cáo khoa học: Bacterial IscU is a well folded and functional single domain protein pdf
... ions, it is probable that the formation of Fe–S clusters is mediated by protein protein interactions. NifS and NifU, the specific proteins involved in the building of Fe–S clusters were originally ... two additional amino acids (GlyAla) at the protein N-terminus. The constructs were expressed in E. coli strain BL21(DE3). For protein expression, the cells were inoculated in Luria–Bertani...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo Y học: Modelling of simple and complex calcium oscillations From single-cell responses to intercellular signalling pdf
... However, cellular Ca 2+ transients also have a spatial dimension. In the cytoplasm of single cells, C a 2+ gradients can be observed when Ca 2+ release from the ER is excited at particular subcellular ... the cells oscillating faster in isolation peak before the slower cells. Thus the intercellular coupling leads to a (near) 1 : 1 entrainment, or synchrony, of the oscillations in adjacent c...
Ngày tải lên: 24/03/2014, 00:21
Báo cáo Y học: Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants docx
... (a1vector)inCHO-K1 cells. Cells were allowed to grow for 10 days before protein expression was analysed. Cell fusion Cell fusion was performed in two individual steps (Fig. 1) by mixing equal number of cells ... each protein subunit. The cell- fusion technology described allows a predefined expression level of each sub- unit. Using SIgA as a model protein we demonstrate that the maj...
Ngày tải lên: 18/03/2014, 01:20
Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
... expression, cells may die during antibiotic selection because of toxic effects of the expressed protein [1]. Also, for a close match of backgrounds, it is better to compare two states of a single cell ... for transport proteins, pools of transfected human embryonic kidney 293 cells showed on ⁄ off mRNA ratios in the order of 100 : 1. Cell pools were also analyzed for regulation of pr...
Ngày tải lên: 23/03/2014, 09:21
Tài liệu Báo cáo khoa học: Evolutionary divergence of valosin-containing protein ⁄cell division cycle protein 48 binding interactions among endoplasmic reticulum-associated degradation proteins ppt
... this fundamental cellular process. For example, valosin-containing protein (VCP) and its yeast homologue cell division cycle protein 48 (Cdc48p), which help to direct polyubiquitinated proteins for ... recombinant proteins and proteins from HeLa cell extract. Fig. S3. Parts (L)–(S) of Fig. 3 to demonstrate Ube4b intrunuclear puncta more clearly. Table S1. Details of the VBM or putativ...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf
... distribution of G 2 ⁄ M cells: the number of metaphase cells with a single DNA mass at the bud neck and the number of anaphase cells were increased slightly in the mutant (metaphase cells: 30.2% in the ... [11]. Keywords atypical protein kinase; casein kinase 2 substrate; cell- cycle phase-dependent degradation; protein protein interactions; Rio1 protein kinase 2 1 Correspondence M....
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Cell surface nucleolin on developing muscle is a potential ligand for the axonal receptor protein tyrosine phosphatase-r ppt
... and incubated with the cells for 1 h at room temperature. Cells were washed, mounted and analyzed as described above. To semipermeabilize cells for intracellular staining, cells were incubated ... human genome. Cell 117, 699–711. 14 Van Vactor D (1998) Protein tyrosine phosphatases in the developing nervous system. Curr Opin Cell Biol 10, 174–181. 15 Stoker A & Dutta R (1998) Prote...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase d and S6K1 and regulating cell growth docx
... intracellular localizations of the human ER and PDIP46/SKAR proteins We examined the intracellular localization of the human ER and PDIP46 ⁄ SKAR proteins fused with enhanced green fluorescent protein ... human ER protein. The screen yielded polymerase d interacting protein 46 or S6K1 Aly ⁄ REF-like target (PDIP46 ⁄ SKAR), a protein possessing one RNA recognition motif (RRM) and being a...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Cell-free translation systems for protein engineering docx
... transformation in cells, and overexpres- sion of the desired protein in cells. Thus, there are limitations associated with using in vivo technology for protein production. Cell- free translation ... engineering. Abbreviations EGFP, enhanced green fluorescent protein; GFP, green fluorescent protein; PDI, protein disulfide isomerase; PURE, protein synthesis using recombinant elements; s...
Ngày tải lên: 19/02/2014, 06:20