transgenic mouse methods and protocols
transgenic mouse methods and protocols
... Deursen Transgenic Mouse Methods and Protocols VOLUME 209 Edited by Marten H. Hofker Jan van Deursen Transgenic Mouse Methods and Protocols Introduction 5 tion. This chapter explains the pros and ... generate transgenic and targeted mouse lines comprise fertile donor females and fertile males for fertilized oocyte Genetic Modifi cation of the Mouse 11 producti...
Ngày tải lên: 11/04/2014, 10:37
Glycoprotein Methods and Protocols - P1
... Methods in Molecular Biology TM HUMANA PRESS Glycoprotein Methods and Protocols Edited by Anthony P. Corfield VOLUME 125 The Mucins Methods in Molecular Biology TM HUMANA ... Mucins Glycoprotein Methods and Protocols Isolation of Large Gel-Forming Mucins 11 Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu- cins were mixed with DNA and...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P2
... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa, NJ. 50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties,...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P3
... in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: The Mucins ... Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi- cation of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The M...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P4
... GI Adherent Mucous Gel Barrier 57 57 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., ... poly- L -lysine for 5 min and dried at room temperature. 3. Methods 3.1. Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections ( see Notes 1–4) This is a rapid and simple method...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P5
... Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... NJ 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. Introduction Most epithelial ... PhosphorImager....
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P6
... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... few separation techniques available to use with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Human...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P7
... Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. ... chapter describes only methods for assessing the heterogeneity of mucin populations with regard to mass and size utilizing rate zonal centrifugation and electron microscopy. Other methods for the absolu...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P8
... 113 113 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., Totowa, NJ 10 Amino Acid Analysis of Mucins Jun X. Yan and ... caramelized and further charcoaled, and the acid hydrolysis results in a black residue. This residue appears to precipitate protein/amino acids, interferes with 114 Yan and...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P9
... Sequencing Glycopeptides 121 121 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., ... 3 and 8) and the length of sequence obtained is dependent on the number and distribution of proline residues. Proline cleaves with reduced efficiency in Edman sequencing (Fig. 1, vessel 1), and ... Kirk, M....
Ngày tải lên: 08/10/2012, 10:25