small gtpases and their regulators, part g
small gtpases and their regulators, part g
...
Biotin-C6-Asp-Asp-Asp-Glu-Glu-Ser-lle-Thr-Arg-Arg
Biotin-C6-Arg-Arg-Arg-Glu-Glu-Glu-Thr-Glu-Glu-Glu Polyamines
Biotin-C6-Lys-Lys-Ala-Leu-Arg-Arg-Gln-Glu-Thr- Ca2+/calmodulin
Val-Asp-Ala-Leu
Two proprietary peptides (Promega)
Cdc2 ... SV40
large T antigen. For each well, mix 0.5-1 ~g of each ShcC expression construct
along with 0.5 ~g of the Gal4-Elk-I plasmid, 2.5 ~g of the Gal4-1u...
small gtpases and their regulators, part a
...
Expression of Epitope (Glu-Glu)-Tagged Ras and Rap Proteins in
Baculovirus-Insect Cell System
The DNA sequence (GAATACATGCCAATGGAA) encoding the
Glu-Glu epitope tag is placed into the 5' ...
SDS-PA GE and Gel Scanning. SDS-PAGE is performed using precast
Daiichi 10-20% polyacrylamide gels purchased from Integrated Separation
Systems or using standard 15% polyacrylamide gels a...
small gtpases and their regulators, part b
... stimulator (GDS), a stimulatory GDP/GTP ex-
change protein for a group of small GTPases including at least Ki-Ras,
Rapl, Rho, and Rac, 1°-13 stimulates the conversion of GDP-Rac to GTP-
Rac and thereby ...
150 nM Smg-GDS, and 300 nM Rho-GDI.
Assay for f~SS]GTPyS-Binding Activity
The [35S]GTPyS-binding activity is assayed by measuring the radioactiv-
ity of [35S]GTPTS bound to...
small gtpases and their regulators, part c
... nucleotide exchange factors (GEFs)],
and factors that stimulate guanine nucleotide hydrolysis [GTPase-activating
proteins (GAPs)].
To understand the function of GTPases and their cognate factors, ... tzM GGPP in the in vitro translation reaction
containing [35S]methionine (as judged then by a molecular size shift after
SDS-PAGE and autoradiography analysis) or 1/zM GGPP and 0....
small gtpases and their regulators, part d
... GAC'~FCT AGA'~GA TcciccG GG~'~'~'IA~
PKH3
BamHI EcoRI ClaI
iGGA TCCUGAA TFCtAtAT CGA T i
PKMyc
Sinai
iNheI Notl EcoRI ClaI
CGG ~3CT AGC t GG~3 CGG CCG C~lqGAA TTCnATC GAT ...
pKFLAG
BamHI EeoRI ClaI Sill
tGGA TCC~tGAA TI"C~AtAT CGA qtGG CCG CCA TGG CC~
PRKT-GFP
BamHI EcoRI ClaI
~~GA GAA ~TC
pKNzz
BamHI Notl EeoRI ClaI
~GCIG CGG CCG CbA ATT C~A~
p...
small gtpases and their regulators, part e
... motifs;
GTP, guanosine triphosphate; Mg 2+, divalent magnesium ion; switch f-II: regions undergoing
large conformational changes during GTP binding and hydrolysis.
effector recognition (Fig. 2). ... and perinuclear Golgi
region). (B) GFP-Rab1-Q67L (ER, punctate VTCs, and perinuclear Golgi region). (C) GFP-
Rab1-S25N (VTCs/fragmented Golgi membranes, ER,
and cytoplasm).
(D) GFP-R...
small gtpases and their regulators, part f
...
ATGGCTTCTAGCTATCCTTATGACGTCCCTGACTATGCCAGCCTCGGA GGATCCCCGGGAATTCCCGGGGATCC pZBRII
I~ M A S S Y P Y D V P D Y A S L G
Sail Ndel Kpnl Sacll BamHI Sscll
GGACCTTCTAGGTCGACCCATATGGTTAACGGTACCCGCGGATCC ... +'" ]
(3235)
Notl (2635)
ATGGCTTCTAGCTATCCTTATGACGTCCCTGAC
k M A S S Y P Y D V P D
BamHI
TATGCCAGCCTCGGA GGACCTTCTAGCGGATCC
k y A S L G G P S S G S
(~~r (eag)
M13 Or...
SMALL-SCALE MINES, THEIR CUMULATIVE ENVIRONMENTAL IMPACTS AND DEVELOPING COUNTRIES BEST PRACTICE GUIDELINES FOR WATER MANAGEMENT
...
(dry-pit mining, wet pit mining, bar skimming and mining of pits on adjacent floodplains or river
terraces), coal mining, gold mining/panning and alluvial gold deposits, clay mining and peat extraction. ... management strategies. These strategies must be
relevant, understandable, affordable, be aimed at maintaining a balance between encouraging economic
developments and preserving...
Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx
... of RGS1 and RGS2 among all the regulators of G- protein sig-
nalling tested. Real-time PCR confirmed modulation of RGS1 and RGS2.
In contrast to RGS2, which was always downregulated, RGS1 mRNA ... modulates RGS1 and RGS2 in a
similar manner. However, the TLR3 ligand poly(I:C) permanently upregu-
lates RGS1 and RGS2 expression indicating a different modulation by the
MyD88- and TRIF-...
Tài liệu Báo cáo khoa học: The small heat shock proteins and their role in human disease pptx
... folding competent intermedi-
ates and conferring enhanced stress resistance on cells
by suppressing aggregation of denaturing proteins,
actions associated with oligomerization and subunit
exchange ... addition of sugars to pro-
teins, is enhanced in rat and human lenses during dia-
betes, causing protein cross-linking and advanced
glycation end-products (AGE), a change engendered
by m...