small gtpases and their regulators, part g
small gtpases and their regulators, part g
... Biotin-C6-Asp-Asp-Asp-Glu-Glu-Ser-lle-Thr-Arg-Arg Biotin-C6-Arg-Arg-Arg-Glu-Glu-Glu-Thr-Glu-Glu-Glu Polyamines Biotin-C6-Lys-Lys-Ala-Leu-Arg-Arg-Gln-Glu-Thr- Ca2+/calmodulin Val-Asp-Ala-Leu Two proprietary peptides (Promega) Cdc2 ... SV40 large T antigen. For each well, mix 0.5-1 ~g of each ShcC expression construct along with 0.5 ~g of the Gal4-Elk-I plasmid, 2.5 ~g of the Gal4-1u...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part a
... Expression of Epitope (Glu-Glu)-Tagged Ras and Rap Proteins in Baculovirus-Insect Cell System The DNA sequence (GAATACATGCCAATGGAA) encoding the Glu-Glu epitope tag is placed into the 5' ... SDS-PA GE and Gel Scanning. SDS-PAGE is performed using precast Daiichi 10-20% polyacrylamide gels purchased from Integrated Separation Systems or using standard 15% polyacrylamide gels a...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part b
... stimulator (GDS), a stimulatory GDP/GTP ex- change protein for a group of small GTPases including at least Ki-Ras, Rapl, Rho, and Rac, 1°-13 stimulates the conversion of GDP-Rac to GTP- Rac and thereby ... 150 nM Smg-GDS, and 300 nM Rho-GDI. Assay for f~SS]GTPyS-Binding Activity The [35S]GTPyS-binding activity is assayed by measuring the radioactiv- ity of [35S]GTPTS bound to...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part c
... nucleotide exchange factors (GEFs)], and factors that stimulate guanine nucleotide hydrolysis [GTPase-activating proteins (GAPs)]. To understand the function of GTPases and their cognate factors, ... tzM GGPP in the in vitro translation reaction containing [35S]methionine (as judged then by a molecular size shift after SDS-PAGE and autoradiography analysis) or 1/zM GGPP and 0....
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part d
... GAC'~FCT AGA'~GA TcciccG GG~'~'~'IA~ PKH3 BamHI EcoRI ClaI iGGA TCCUGAA TFCtAtAT CGA T i PKMyc Sinai iNheI Notl EcoRI ClaI CGG ~3CT AGC t GG~3 CGG CCG C~lqGAA TTCnATC GAT ... pKFLAG BamHI EeoRI ClaI Sill tGGA TCC~tGAA TI"C~AtAT CGA qtGG CCG CCA TGG CC~ PRKT-GFP BamHI EcoRI ClaI ~~GA GAA ~TC pKNzz BamHI Notl EeoRI ClaI ~GCIG CGG CCG CbA ATT C~A~ p...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part e
... motifs; GTP, guanosine triphosphate; Mg 2+, divalent magnesium ion; switch f-II: regions undergoing large conformational changes during GTP binding and hydrolysis. effector recognition (Fig. 2). ... and perinuclear Golgi region). (B) GFP-Rab1-Q67L (ER, punctate VTCs, and perinuclear Golgi region). (C) GFP- Rab1-S25N (VTCs/fragmented Golgi membranes, ER, and cytoplasm). (D) GFP-R...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part f
... ATGGCTTCTAGCTATCCTTATGACGTCCCTGACTATGCCAGCCTCGGA GGATCCCCGGGAATTCCCGGGGATCC pZBRII I~ M A S S Y P Y D V P D Y A S L G Sail Ndel Kpnl Sacll BamHI Sscll GGACCTTCTAGGTCGACCCATATGGTTAACGGTACCCGCGGATCC ... +'" ] (3235) Notl (2635) ATGGCTTCTAGCTATCCTTATGACGTCCCTGAC k M A S S Y P Y D V P D BamHI TATGCCAGCCTCGGA GGACCTTCTAGCGGATCC k y A S L G G P S S G S (~~r (eag) M13 Or...
Ngày tải lên: 11/04/2014, 10:32
SMALL-SCALE MINES, THEIR CUMULATIVE ENVIRONMENTAL IMPACTS AND DEVELOPING COUNTRIES BEST PRACTICE GUIDELINES FOR WATER MANAGEMENT
... (dry-pit mining, wet pit mining, bar skimming and mining of pits on adjacent floodplains or river terraces), coal mining, gold mining/panning and alluvial gold deposits, clay mining and peat extraction. ... management strategies. These strategies must be relevant, understandable, affordable, be aimed at maintaining a balance between encouraging economic developments and preserving...
Ngày tải lên: 05/09/2013, 09:08
Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx
... of RGS1 and RGS2 among all the regulators of G- protein sig- nalling tested. Real-time PCR confirmed modulation of RGS1 and RGS2. In contrast to RGS2, which was always downregulated, RGS1 mRNA ... modulates RGS1 and RGS2 in a similar manner. However, the TLR3 ligand poly(I:C) permanently upregu- lates RGS1 and RGS2 expression indicating a different modulation by the MyD88- and TRIF-...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: The small heat shock proteins and their role in human disease pptx
... folding competent intermedi- ates and conferring enhanced stress resistance on cells by suppressing aggregation of denaturing proteins, actions associated with oligomerization and subunit exchange ... addition of sugars to pro- teins, is enhanced in rat and human lenses during dia- betes, causing protein cross-linking and advanced glycation end-products (AGE), a change engendered by m...
Ngày tải lên: 19/02/2014, 18:20