small gtpases and their regulators, part b
small gtpases and their regulators, part b
... Fig. 3. To obtain reliable data, the presence of equal amount of small < /b> GTPases < /b> in each lane should be confirmed. This can be carried out by reprobing the blot with antibodies to the small < /b> GTPase ... diluted fivefold with buffer B and < /b> applied to a CM-Sepharose column (1.5 x 20 cm) equilibrated with buffer B. Elution is performed with 50 ml of buffer B foll...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part a
... of buffer B, [2] PURIFICATION OF BACULOVIRUS-EXPRESSED Ras AND < /b> Rap, 19 30 vol of buffer B containing 0.5% (w/v) sodium cholate, 30 vol of buffer B containing 500 mM NaCI, and < /b> 30 vol of buffer ... washed with 30 vol of buffer B, 30 vol of buffer B containing 0.5% Nonidet P-40 (NP-40), 30 vol of buffer B containing 500 mM NaCI, and < /b> 30 vol of buffer B. Unpro...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part c
... expression and < /b> the particular property of Rab5 to be eluted by CHAPS, the purity is over 90%. Purified unmodified Rab5 efficiently binds GTP and < /b> GDP but, as expected, Rab-GDI does not inhibit [35S]GTP3~S ... modified Rab5 by Mono Q column chromatogra- phy. (A) Chromatography profile and < /b> [35S]GTP3,S binding activity in the presence (O) and < /b> in the absence (0) of...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part d
... 6 4 I. G. Macara and < /b> W. H. Brondyk, Methods Enzymol. 257, 117 (1995). B. R. Bochner and < /b> B. N. Ames, J. Biol, Chem. 257, 9759 (1982). 6E. S. Burstein and < /b> I. G. Macara, Biochem. J. 282, ... temperature for I hr. Block the membrane in binding buffer containing 5% (w/v) milk at 4 ° for 1 hr in a small < /b> container. To block nonspecific GTP binding, incubate t...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part e
... applicable to other members of the larger superfamily of Ras-like GTPases.< /b> Given the abundance of both Rab and < /b> ARF GTPases < /b> and < /b> the intense interest of the cell biology community in their < /b> function, ... Chiariello, M. Bifulco, C. B. Bruni, and < /b> C. Bucci, J. Biol. Chem. 272, 4391 (1997). 4 p. j. Casey and < /b> M. C. Seabra, J. BioL Chem. 271, 528...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part f
... shown. Base pairs in pBABE-Puro and < /b> pZIP-NeoSV(X) 1 have been renumbered from Refs. 3 and < /b> 6, respectively, to begin at the BamHI site, while base pairs in pDCR are numbered beginning at the Sail ... Anti-HA and < /b> anti-Myc antibodies are available from InVitrogen (Carlsbad, CA), Boehringer-Mannheim/Roche (Indianapolis, IN), Berkeley Antibody Company (BAbCo, Richmond,...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part g
... Diego, CA) and < /b> then analyzed by Western blot with the same antibodies. (A and < /b> B) Filters were probed with ShcA antibody (1 tag/ml) or ShcC antibody (1:500) as indicated, using anti-rabbit Ig-HRP ... using anti- rabbit HRP (Fig. 2A and < /b> B) versus protein A-HRP (Fig. 2C and < /b> D) as the secondary detection reagent, in Fig. 2A and < /b> B, the heavy chain band obs...
Ngày tải lên: 11/04/2014, 10:32
applications of chimeric genes and hybrid proteins, part b
... receptor ectodomains can be used as soluble probes to detect their < /b> ligands.lT2 When producing soluble receptor or ligand affinity probes, it has been common to produce the probe as a fusion protein ... R. G. Chubet and < /b> B. L. Brizzard, BioZ’echniques 20,136 (1996); (9) T. P. Hopp, K. S. Prickett, V. L. Price, R. T. Libby, C. J. March, D. P. Cerretti, D. L. Urdal, and < /b...
Ngày tải lên: 10/04/2014, 11:02
quinones and quinone enzymes, part b
... Biomembranes [Part < /b> K: Membrane Biogenesis: Assembly and < /b> Targeting (Prokaryotes, Mitochondria, and < /b> Chloroplasts)] Edited by Sidney Fleischer and < /b> Becca Fleischer Volume 98. Biomembranes (Part < /b> L: Membrane ... Techniques Edited by Peter K. Vogt and < /b> Inder M. Verma Volume 255. Small < /b> GTPases < /b> and < /b> Their < /b> Regulators (Part < /b> A: Ra...
Ngày tải lên: 11/04/2014, 10:23
rna polymerase and associated factors, part b
... DER, AND < /b> ALAN HALL VOLUME 256. Small < /b> GTPases < /b> and < /b> Their < /b> Regulators (Part < /b> B: Rho Family) Edited by W. E. BALCH, CHANNING J. DER, AND < /b> ALAN HALL VOLUME 257. Small < /b> GTPases < /b> and < /b> Their < /b> Regulators (Part < /b> ... Structure, and < /b> Molecu- lar Biology) Edited by JERE P. SEGREST AND < /b> JOHN J. ALBERS VOLUME 129...
Ngày tải lên: 11/04/2014, 10:30