small gtpases and their regulators, part a
small gtpases and their regulators, part a
... all human tumors. Rap proteins (Rap 1 A, Rap l B, and Rap2) are Ras-related GTPases that share 53% amino acid homology with Ras and are able to antagonize the effects of oncogenic Ras in ... modifications comprise farnesylation (Ras) or geranyl- geranylation (Rap) of the cysteine residue, removal of the AAX amino acids, and carboxymethylationY In addition H-Ras, N-Ras, and...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part b
... fractions 16-40 and 53-56. When each fraction is assayed for the NADPH oxidase activity, two peaks appear in fractions 17-23 as a major peak and in fractions 53-56 as a minor peak. The major ... 75014 Paris, France ALESSANDRA EVA (38), Laboratory of Cellu- lar and Molecular Biology, National Cancer Institute, National Institute of Health, Bethesda, Maryland 20892 LARRY a. F...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part c
... that termi- nate in a CAAX box (C, cysteine; A, aliphatic amino acid; X, any amino acid). The enzyme that modifies Yptlp and Sec4p, called Rab or type-II geranylgeranyltransferase (GGTase ... SOLDATI, A. BARBARA DIRAC-SVEJSTRUP, and SUZANNE R. PFEFFER Introduction This chapter describes the purification of canine Rab9 after expression in Escherichia coli, and the small- sc...
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small gtpases and their regulators, part d
... PstI SalI XbaI BamHI EcoRI ClaI ~'CTG CAG"GTC GAC'~FCT AGA'~GA TcciccG GG~'~'~'IA~ PKH3 BamHI EcoRI ClaI iGGA TCCUGAA TFCtAtAT CGA T i PKMyc Sinai iNheI ... Fujioka, K. Kaibuchi, and Y. Takai, Oncogene 6, 515 (1991). 2 T. Mizuno, K. Kaibuchi, T. Yamamoto, M. Kawamura, T. Sakoda, H. Fujioka, Y. Matsuura, and Y. Takai, Proc. Natl. Acad. Sci. U.S ....
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part e
... Valap may be used to seal coverslips. Valap is a wax-based material made by mixing Vasoline, lanolin, and paraf- fin in a 1:1:1 ratio and liquefies when heated to 40-50 °. On cooling, Valap ... Valap solidifies and creates a hardened, air- and watertight seal around the specimen. As another alternative, ProLong antifade reagent (Molecular Probes) may be used instead of Aqu...
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small gtpases and their regulators, part f
... in our laboratories. For each plasmid we have identified, when available, the promoter, mammalian origins of replication, bacterial and mammalian selectable markers, retroviral packaging sequences, ... common. Although the GFP moiety, like HA and Myc, is detectable with commercially available anti- bodies and can act as a standard epitope tag, it also permits the direct visualiza...
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small gtpases and their regulators, part g
... polyclonal Goat polyclonal Santa Cruz Biotechnology hShcA (aa 359473) hShcA (aa 359-473) mShcC (aa 239-374) hShcA (aa 454-473) hShcA (aa 366-473) hShcA (aa 366-473) hShcA (aa 2-20) hShcC (aa ... scintillation vials. Add scintillation lluid to the vials and count. 12. Phosphorhnager analysis: Alternatively, the SAM e membrane may remain intact and the intact SAM e membrane may...
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applications of chimeric genes and hybrid proteins, part a
... A A B lacA' lacY lacZ'trpA' J l~l~'fJffffA ~ N S cA cI RA N J ~r 'trpA'lacZ lacY lacA'~ Ac S cI R placMu50 trpA' lacZ lacY lacA' ~'Jfffff~\~ ... imm21 lacZYA ' Transcriptional AplacMu54 ~" AplacMu53 d immA, Kan R lacZYA ' Transcriptional AplacMu55 " AplacMul I immA lac "ZYA' Translational...
Ngày tải lên: 10/04/2014, 11:02
quinones and quinone enzymes, part a
... Verma Volume 255. Small GTPases and Their Regulators (Part A: Ras Family) Edited by W. E. Balch,Channing J. Der, and Alan Hall Volume 256. Small GTPases and Their Regulators (Part B: Rho Family) Edited ... Polymerases and Associated Factors (Part C) Edited by Sankar L. Adhya and Susan Garges Volume 371. RNA Polymerases and Associated Factors (Part D) Edited by San...
Ngày tải lên: 11/04/2014, 10:23
rna polymerase and associated factors, part a
... VOGT AND INDER M. VERMA VOLUME 255. Small GTPases and Their Regulators (Part A: Ras Family) Edited by W. E. BALCH, CHANNING J. DER, AND ALAN HALL VOLUME 256. Small GTPases and Their Regulators ... P450 (Part B) Edited by ERIC F. JOHNSON AND MICHAEL R. WATERMAN VOLUME 273. RNA Polymerase and Associated Factors (Part A) Edited by SANKAR ADHYA VOLUME 274. RNA...
Ngày tải lên: 11/04/2014, 10:30