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small gtpases and their regulators, part a

small gtpases and their regulators, part a

small gtpases and their regulators, part a

... all human tumors. Rap proteins (Rap 1 A, Rap l B, and Rap2) are Ras-related GTPases that share 53% amino acid homology with Ras and are able to antagonize the effects of oncogenic Ras in ... modifications comprise farnesylation (Ras) or geranyl- geranylation (Rap) of the cysteine residue, removal of the AAX amino acids, and carboxymethylationY In addition H-Ras, N-Ras, and K-Ras (A) ... in the baculovirus-insect cell system are processed in the same way as in mammalian cells. H-Ras is farnesylated and palmitoylated, s K-Ras is farnesylated, ~) and Rap is geranylgeranylated,...
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small gtpases and their regulators, part b

small gtpases and their regulators, part b

... fractions 16-40 and 53-56. When each fraction is assayed for the NADPH oxidase activity, two peaks appear in fractions 17-23 as a major peak and in fractions 53-56 as a minor peak. The major ... 75014 Paris, France ALESSANDRA EVA (38), Laboratory of Cellu- lar and Molecular Biology, National Cancer Institute, National Institute of Health, Bethesda, Maryland 20892 LARRY a. FEIG ... eXVRZSSION AND PURIFICATION [31 contains heterotrimeric GTPases and the second peak contains small GTPases including Rac2. When each fraction is assayed for the NADPH oxidase activity, a single peak...
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small gtpases and their regulators, part c

small gtpases and their regulators, part c

... that termi- nate in a CAAX box (C, cysteine; A, aliphatic amino acid; X, any amino acid). The enzyme that modifies Yptlp and Sec4p, called Rab or type-II geranylgeranyltransferase (GGTase ... SOLDATI, A. BARBARA DIRAC-SVEJSTRUP, and SUZANNE R. PFEFFER Introduction This chapter describes the purification of canine Rab9 after expression in Escherichia coli, and the small- scale and ... small- scale reactions should include 1/xM GGPP and 0.1/xM [3H]GGPP, and be followed by SDS-PAGE and fluorography. Another small- scale prenylation assay used to assess prenylatability of a...
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small gtpases and their regulators, part d

small gtpases and their regulators, part d

... PstI SalI XbaI BamHI EcoRI ClaI ~'CTG CAG"GTC GAC'~FCT AGA'~GA TcciccG GG~'~'~'IA~ PKH3 BamHI EcoRI ClaI iGGA TCCUGAA TFCtAtAT CGA T i PKMyc Sinai iNheI ... Fujioka, K. Kaibuchi, and Y. Takai, Oncogene 6, 515 (1991). 2 T. Mizuno, K. Kaibuchi, T. Yamamoto, M. Kawamura, T. Sakoda, H. Fujioka, Y. Matsuura, and Y. Takai, Proc. Natl. Acad. Sci. U.S .A. ... bacteria that directly affect the activity of mammalian Rho GTPases. We very much hope that this and the accompanying volumes covering the Ras family (Volumes 332 and 333) and the small GTPases...
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small gtpases and their regulators, part e

small gtpases and their regulators, part e

... Valap may be used to seal coverslips. Valap is a wax-based material made by mixing Vasoline, lanolin, and paraf- fin in a 1:1:1 ratio and liquefies when heated to 40-50 °. On cooling, Valap ... Valap solidifies and creates a hardened, air- and watertight seal around the specimen. As another alternative, ProLong antifade reagent (Molecular Probes) may be used instead of Aqua PolyMount to ... PC12 cells and released in response to various agonists in an extracellular Ca2+-dependent manner. 29,3° 2~ T. D. Wakade, S. V. Bhave, A. S. Bhave, R. K. Malhotra, and A. R. Wakade, J. Biol....
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small gtpases and their regulators, part f

small gtpases and their regulators, part f

... in our laboratories. For each plasmid we have identified, when available, the promoter, mammalian origins of replication, bacterial and mammalian selectable markers, retroviral packaging sequences, ... common. Although the GFP moiety, like HA and Myc, is detectable with commercially available anti- bodies and can act as a standard epitope tag, it also permits the direct visualization of ... signal through Elk-1. Similarly, Preface As with the Rho and Rab branches of the Ras superfamily of small GTPases, research interest in the Ras branch has continued to expand dramatically...
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small gtpases and their regulators, part g

small gtpases and their regulators, part g

... polyclonal Goat polyclonal Santa Cruz Biotechnology hShcA (aa 359473) hShcA (aa 359-473) mShcC (aa 239-374) hShcA (aa 454-473) hShcA (aa 366-473) hShcA (aa 366-473) hShcA (aa 2-20) hShcC (aa ... scintillation vials. Add scintillation lluid to the vials and count. 12. Phosphorhnager analysis: Alternatively, the SAM e membrane may remain intact and the intact SAM e membrane may be analyzed ... than appreciated previously. In particular, while the Raf/MEK/ERK kinase cascade remains a key signaling pathway activated by Ras, it is now appreciated that an increasing number of non-Raf...
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applications of chimeric genes and hybrid proteins, part a

applications of chimeric genes and hybrid proteins, part a

... A A B lacA' lacY lacZ'trpA' J l~l~'fJffffA ~ N S cA cI RA N J ~r 'trpA'lacZ lacY lacA'~ Ac S cI R placMu50 trpA' lacZ lacY lacA' ~'Jfffff~\~ ... imm21 lacZYA ' Transcriptional AplacMu54 ~" AplacMu53 d immA, Kan R lacZYA ' Transcriptional AplacMu55 " AplacMul I immA lac "ZYA' Translational AplacMu5" ... AplacMu3f imm21 lac "ZYA' Translational AplacMul 3" AplacMu9 j irnmA, Kan R lac "ZYA' Translational AplacMul5" '~ imm, phage immunity; Kan R, kanamycin...
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quinones and quinone enzymes, part a

quinones and quinone enzymes, part a

... VermaVolume 255. Small GTPases and Their Regulators (Part A: Ras Family)Edited by W. E. Balch,Channing J. Der, and Alan HallVolume 256. Small GTPases and Their Regulators (Part B: Rho Family)Edited ... Polymerases and Associated Factors (Part C)Edited by Sankar L. Adhya and Susan GargesVolume 371. RNA Polymerases and Associated Factors (Part D)Edited by Sankar L. Adhya and Susan GargesVolume 372. ... and William S. HancockVolume 272. Cytochrome P450 (Part B)Edited by Eric F. Johnson and Michael R. WatermanVolume 273. RNA Polymerase and Associated Factors (Part A) Edited by Sankar AdhyaVolume...
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rna polymerase and associated factors, part a

rna polymerase and associated factors, part a

... VOGT AND INDER M. VERMA VOLUME 255. Small GTPases and Their Regulators (Part A: Ras Family) Edited by W. E. BALCH, CHANNING J. DER, AND ALAN HALL VOLUME 256. Small GTPases and Their Regulators ... P450 (Part B) Edited by ERIC F. JOHNSON AND MICHAEL R. WATERMAN VOLUME 273. RNA Polymerase and Associated Factors (Part A) Edited by SANKAR ADHYA VOLUME 274. RNA Polymerase and Associated Factors ... bending appears to be to facilitate contact between the activator and RNAP. [An alternative method of achiev- ing contact between a DNA-binding activator and RNAP bound at a (T4 late) promoter...
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