recombinant dna part g
recombinant dna part g
... PREPARING AND USING AGAROSE MICROBEADS
13
[2]
Preparing and Using
Agarose Microbeads
By MICHAEL KOOB
and WACLAW SZYBALSKI
Introduction
Protocols for preparing and manipulating large DNA molecules ... detection of DNA and RNA, enzymes for manipu-
lating DNA, reporter genes, and new vectors for cloning genes. Volume
217 includes vectors for expressing cloned genes, mutagenesis, i...
recombinant dna part a
...
packaging into h phage particles, and they replicate as plasmids because
of their ColE1 DNA segments. Their chief advantage is the fact that pJC
DNA ligated to foreign DNA can be packaged
in ...
(fl-galactosidase) gene. Substitution of foreign DNA in place of the
lacPOZ
DNA segment makes the phage
lacO
Growth on a
lac +
bacterial
strain on plates containing a chromogenic n...
recombinant dna part b
...
HaelII
GGCmC GGmCC j, k
HaplI
CmCGG e, l
Hha
I GmCGC f, j
HpalI
mCCGG C~"CGG j, l
MspI
CmCGG mCCGG I, m
Pst
I mCTGCAG n
Pvu
II mCAGCTG n
SalI
GT~CGAC d, e
SrnaI
CCmCGGG e, ... u
Taql
TmCGA o
Xhol
CTmCGAG d, e
Xma
I CCmCGGG u
Barn
HI GGmATCC
g, p
Bglll
AGmATCT p
DpnI
GmATC c o, q
Dpn
II GmATC q
EcoRI
GAmATTC r
Fnu
E1 GmATC s
HindlI
GTPyPumAC k...
recombinant dna part c
... BamH1
gamma genes on
17 Kb Bam H1
fragment
L
Anneal fragments withT4 DNA l igase
Parental Phages
red+y +
High Molecular Weight Eukaryotic DNA
Digest DNA with Purify 15-20 Kb
Bam H1, Bgl ... lysogens
but phages grow on RecA strains
Phages deleted in red and gamma genes
Phages grow on P2 Lysogens but growth
is restricted on Rec A strains
FIG. 1. Schematic diagram outlining...
recombinant dna part d
... ~.EC AGC T&TGACCAT- 3 *
m.mmtmuumlmn~ll
3 ~ -TTTGTCGATAC TGGTAC TAATGCGGTTCGA ACGTACGGACGTCC AGC TGAGATC TCCTAGGGGCCCATGGC TCGACCTTAAGTGACCGGC AGC AAAATGTT- 5 '
F-at, raJld
FzG. 2. ... ' - AAAC AGCTATGACCATGATTACGCC AAGCTTGCATGCCTGCAGGTCGAC TC TAGAGGATCCCCGGGTACCGAGCTCGAATTC AC TGGCCGTCGTTTTAC AA- 3 '
I-atrana
illllillllllllllil
31 -GTGACCGGCAGCAAAATG-5 '
maste...
recombinant dna part e
... of high molecu-
lar weight DNA by base-pairing between dG-tails, the yield of linker DNA
from agarose gels is generally poor.
cDNA Cloning
Steps in the construction of cDNA-plasmid recombinants ...
H/ndlll ~GG G ¢/~ , ~- -T T A J DIGESTION
ANNEALING OF OLIGO dG '
Hindm Xho[ 8BamHl
TAILED LINKER; CYCLIZATION
WITH E.co/ /DNA LIGASE /
~ .G~ ~ ~ __~- REPLACEMENT OF RNA STRAN...
recombinant dna part f
...
AGCAN~TGATCGCGA 11.7
M.EcoB/M.TaqII
AGCANsTGATCGGTC 11.7
M.EcoB/M.XbaI
AGCANsTGATCTAGA 11.7
M.AccIII/M.AccIII
TCCGGATCCGGA 12.0
M.NruI/M.NruI
TCGCGATCGCGA 12.0
M.TaqII/M.TaqII
GACCGATCGGTC ...
TCCGGATCGCGA 11.0
M.AcclII/M.TaqII
TCCGGATCGGTC 11.0
M.AccIII/M.XbaI
TCCGGATCTAGA 11.0
M.NruI/M.XbaI
TCGCGATCTAGA 11.0
M. TaqlI/M.XbaI
GACCGATCTAGA 11.0
M.AccIII/M.EcoB
TCCGGATCANs...
recombinant dna part h
...
GTCGRCGGATCCCCGGGCCCATGGCCGCGGTCGCGTATATGCGCGCGCGCARRGCTGGCGGCCGC
I I I ! I I i I i I I i ! I ! I I I ! I ! I
SP6
promoter
A GCTI'GA GTATTCTITI'A GTGTG A G CTA A ATAGCTFG 6C GTAATC ATG GTC ...
AAOATTCACGAATTCTAGCAC, TTGTGTTACACTAGTGATTGTTGCATTTTACACAATACT
41
5~ SDI 4zo
GAATATACTAOAGATTTTTAACACAAAAAGCGAGGCTTTCCTGCGAAAGGAGGTGACACG
480
COCTTGCAGGATTCGGGCTTTAAAAAGAAAGATAGAT...
recombinant dna part i
... (1981)] was amplified using the primers UG142 (5'-
GGTCTATCACCCTATTAACCAC-3') and UG143 (5'-CTGTTAAAAGTGCATACCGC-
CA-3') and sequenced using UG142. The arrows indicate the ... and mapping genes, and methods for transforming animal and plant
cells. Volume 218 includes methods for sequencing DNA, PCR for ampli-
fying and manipulating DNA, methods for detecting DNA- pr...
PART G - CONSTRUCTION EQUIPMENT ppt
... Subsection G3 02.07 of this Manual.
G4 03 –MILLING MACHINES
Revised: January 2004
G- 25
SECTION G4 03 – MILLING MACHINES
G4 03.01 General. The terms cold planing, grinding, profiling, and milling refer ... making this type of grader useful for working on side slopes and ditches.
Figure G- 13 shows a typical motor grader.
G3 01 –MOTOR GRADERS
Revised: January 2004
G- 12
F...
Từ khóa:
- molecular genetics recombinant dna amp genomic technology
- general strategy for genetic constructions by pcr without recombinant dna cloning
- genetics recombinant dna technology and microarrays
- g gene into dna plasmid form recombinant dna
- điện di dna trên gel agarose
- dna cây gỗ sưa
- tach chiet dna cua gan lon
- dna methylation genotype and gene expression