recombinant dna part c
recombinant dna part c
... dlII 6 ° chi C right arm and has the poly- linker sequence TCTAGAATTCAAGCTTGGATCCTCGAGCTCTAGA cloned into the Xba sites. This phage is a vector for EcoRI, HindlIl, BamHI, XhoI, SacI, and ... bromide per milliliter. Nick-translated EcoRI-cuthDNA, BamHl-cut 1059 DNA, and a clone ofunc54 DNA cut with BamHl were included as size markers. Fractions 3, 4, and 5 contain 15-20 kb...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part a
... "¢ehicle DNA can then be ligated into a recombinant circle with the passenger DNA, or the passenger DNA can be cyclized to yield molecules which generally cannot replicate, but the vehicle cannot ... can easily be screened for a given DNA sequence by nucleic acid hybridization. 43 Second, in vitro packaging of recombinant DNA molecules provides a very efficient means...
Ngày tải lên: 11/04/2014, 10:25
recombinant dna part b
... EcoRII CmC(A)GG h, i (T) Hae II PuGmCGCPy e, f HaelII GGCmC GGmCC j, k HaplI CmCGG e, l Hha I GmCGC f, j HpalI mCCGG C~ "CGG j, l MspI CmCGG mCCGG I, m Pst I mCTGCAG n Pvu ... II mCAGCTG n SalI GT~CGAC d, e SrnaI CCmCGGG e, u Taql TmCGA o Xhol CTmCGAG d, e Xma I CCmCGGG u Barn HI GGmATCC g, p Bglll AGmATCT p DpnI GmATC c o, q Dpn II GmATC q...
Ngày tải lên: 11/04/2014, 10:25
recombinant dna part d
... VECTORS FOR CLONING DNA [2] B A r pKUN9 CCAAGC T TGGCTGCAGGTCGACGGATC CCCGGGAAT T C A (3898 bp) Hindm Pstl fiomH| E¢oRI Hint I/ pKUN19 CCAAGC T TGC ATGCC TGC AGGTCGAC TC TAGAGGATCCCCGGGTACCGAGCTCGAATTCA ... reverael(+)-prlmer 5 ~ - ~.EC AGC T&TGACCAT- 3 * m.mmtmuumlmn~ll 3 ~ -TTTGTCGATAC TGGTAC TAATGCGGTTCGA ACGTACGGACGTCC AGC TGAGATC TCCTAGGGGCCCATGGC TCGACCTTAAGTGACCGGC...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part e
... of the vector primer under the conditions described in the cDNA Cloning section "Pilot-scale reac- tion." Calculate the percent conversion to cDNA from the amounts of cDNA synthesized ... products are closed circular cDNA-vector recombinants. Reagents HindlII-cut, C- tailed cDNA : mRNA-vector (Step 3) dG-tailed linker DNA (0.3 pmol//.d) 5x hybridization buffer: 50 mM Tri...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part f
... *M.TaqI/M.TaqI TCGATCGA 8.0 *M.CIaI/M.MboII ATCGATCTTC 9.0 M.AccIII/M.ClaI TCCGGATCGAT 9.7 M.AccIII/M.MboII TCCGGATCTTC 9.7 M.ClaI/M.NruI ATCGATCGCGA 9.7 M.CIaI/M.TaqII ATCGATCGGTC 9.7 M.ClaI/M.XbaI ... M.EcoB/M.MboII AGCAN~TGATCTTC 9.9 *M.ClaI/M.ClaI ATCGATCGAT 10.0 *M.MbolI/M.MboII GAAGATCTTC 10.0 M.AccIII/M.NruI TCCGGATCGCGA 11.0 M.AcclII/M.TaqII TCCGGATCGGTC 11.0 M.A...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part g
... cally obtained with agarose plugs. Although the details in the protocol given are for the preparation of Escherichia coli and Saccharomyces cere- visiae genomic DNA, these procedures can ... performance of colony hybridization using a labeled probe specific for the cloned DNA. ~ Colony hybridization would be especially useful if the cloning efficiency is expected to be low. Cloning...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part h
... ATCAGATCCGCTATCCTOTCTTACAACTTOOCTOTTOTAAACTTTOAAAATOCATTAOOA 120 AATTAACCTAATTCAAGCAAGATTATOAO(]TTTT(]AACCAAATTGGAAAAAGOTTCAGTC l~ 18o 0TGACAGGCCGCCATATOI'CCCCTATAATACGGATTOTGGCOGATGTCACTTCOTACATA ... AAOATTCACGAATTCTAGCAC, TTGTGTTACACTAGTGATTGTTGCATTTTACACAATACT 41 5~ SDI 4zo GAATATACTAOAGATTTTTAACACAAAAAGCGAGGCTTTCCTGCGAAAGGAGGTGACACG 480 COCTTGCAGGATTCGGGCTTTAAAAAGAAAGATAG...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part i
... (5'- GGTCTATCACCCTATTAACCAC-3') and UG143 (5'-CTGTTAAAAGTGCATACCGC- CA-3') and sequenced using UG142. The arrows indicate the location of point mutational differences between ... Science 245, 1066 (1989)] was amplified using primer A (5'- CTGTGTCTGTAAACTGATGGCTA-3') and primer B (5'-GTCTTCTTCGTTAATTTCTT- CAC-3'). The PCR mix included 0.1/xl [c~ -3...
Ngày tải lên: 11/04/2014, 10:27
Unit 9: Part C Listening
... hunting whales? Because they want to catch whales for food, oil, leather and other products. Listen and repeat. mammal, blue whale. Atlantic, Pacific, krill , whaling, migrate, conservation ... 3. According to the listening passage, what are the food feeding grounds for whales? - Cold waters in the North South Atlantic Ocean and the North and South Pacific. 4. What is the ... for p...
Ngày tải lên: 24/06/2013, 01:28