recombinant dna part a
recombinant dna part a
... of yeast DNA, or cer- tain prokaryotic DNAs, in yeast. A vehicle for recombinant DNA and a transformation procedure are also available for Staphylococcus aureus.S2 Many recombinants containing ... combined treatment with nuclease S 1 followed by DNA polymerase I and dNTPs has been employed. 6a& apos;7° Introducing Recombinant DNA into a Host Almost all recombinant...
Ngày tải lên: 11/04/2014, 10:25
recombinant dna part b
... IN RECOMBINANT DNA [4] Sinai Ava I z: : , i, ,u ,, 4 A " 5 FIG. 9. (A) autoradiogram of Bal31 nuclease digestions of SmaI- and AvaI-treated pYT2 DNA. (B) Autoradiogram of Bal31 ... Improp- erly prepared DNA samples will be cleaved poorly, if at all, producing partially digested DNA. In addition to DNA purity, other DNA- associ- ated parameters that affect the prod...
Ngày tải lên: 11/04/2014, 10:25
recombinant dna part c
... phage DNA lambda red and with BamH1 gamma genes on 17 Kb Bam H1 fragment L Anneal fragments withT4 DNA l igase Parental Phages red+y + High Molecular Weight Eukaryotic DNA Digest DNA ... single-stranded form is released from the intact bacterial cell in a filamentous phage particle that can easily be separated from all bacterial and intracellular viral components. All vi...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part d
... -TTTGTCGATAC TGGTAC TAATGCGGTTCGA ACGTACGGACGTCC AGC TGAGATC TCCTAGGGGCCCATGGC TCGACCTTAAGTGACCGGC AGC AAAATGTT- 5 ' F-at, raJld FzG. 2. (A) Genetic organization of plasmid pKUN. pKUN contains ... TAGAGGATCCCCGGGTACCGAGCTCGAATTC AC TGGCCGTCGTTTTAC AA- 3 ' I-atrana illllillllllllllil 31 -GTGACCGGCAGCAAAATG-5 ' master/( -)-prlaler reverael(+)-prlmer 5 ~ - ~.EC AGC T&...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part e
... sample was given two 15-rain washes at room temperature in 6x SSC after the hybrid- ization. An identical sample was given the same treatment and an addi- tional 4-hr wash at room temperature ... Oligo(dG)-Tailed Linker DNA- Mediated Cyclization and Repair of RNA Strand The HindlII-cut, C-tailed cDNA : mRNA-vector is cyclized by DNA ligase and the oligo(dG)-tailed linker DNA that b...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part f
... M.AccIII/M. Taql TCCGGATCGA 7.9 M.EcoB/M.TaqI AGCANsTGATCGA 7.9 M.NruI/M. TaqI TCGCGATCGA 7.9 M.TaqI/M.TaqII TCGATCGGTC 7.9 M.Taql/M.XbaI TCGATCTAGA 7.9 *M.TaqI/M.TaqI TCGATCGA 8.0 *M.CIaI/M.MboII ... M.MboII/M.NruI GAAGATCGCGA 9.7 M.MboII/M. TaqII GAAGATCGGTC 9.7 M.MboII/M.XbaI GAAGATCTAGA 9.7 M.NruI/M. TaqII TCGCGATCGGTC 9.7 M.EcoB/M,CIaI AGCANsTGATCGAT 9.9 M.EcoB/M.M...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part g
... relic DNA and hybridizes strongly to an rDNA probe (data not shown). A similar rate of lysis was observed with isolated protoplasts by Van Daelen et al. 7 and Cheung and Gale. 8 [1] MEGABASE ... small size, agarose microbeads potentially offer sev- eral advantages over agarose blocks, both in ease of handling and in the speed with which the DNA can be prepared and enzymatically...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part h
... ATCAGATCCGCTATCCTOTCTTACAACTTOOCTOTTOTAAACTTTOAAAATOCATTAOOA 120 AATTAACCTAATTCAAGCAAGATTATOAO(]TTTT(]AACCAAATTGGAAAAAGOTTCAGTC l~ 18o 0TGACAGGCCGCCATATOI'CCCCTATAATACGGATTOTGGCOGATGTCACTTCOTACATA 240 ATGGACAGOTGAATAACGAACCACGAAAAAAACTTTAAATTTTTTTCGAAGGGGCCGCAA ... TTGTGTTACACTAGTGATTGTTGCATTTTACACAATACT 41 5~ SDI 4zo GAATATACTAOAGATTTTTAACACAAAAAGCGAGGCTTTCCTGCGAAAGGAGGTGACAC...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part i
... regular agarose gel. The ssDNA is visible after the bromphenol blue has migrated about 2 cm as a discrete fraction migrating ahead of the dsDNA. If a ssDNA fraction is visible by ethidium staining, ... G. Young, Nature (London) 290, 457 (1981)] was amplified using the primers UG142 (5'- GGTCTATCACCCTATTAACCAC-3') and UG143 (5'-CTGTTAAAAGTGCATACCGC- CA-3') and se...
Ngày tải lên: 11/04/2014, 10:27
