quantitative trait loci, methods and protocols
quantitative trait loci, methods and protocols
... CampAngela Cox Quantitative Trait LociVOLUME 195 Methods and Protocols Edited byNicola J. CampAngela Cox Quantitative Trait Loci Methods and Protocols Parametric Linkage Analysis 21Fig. 1. ... appropriate correc-tions are made (1).From: Methods in Molecular Biology: vol. 195: Quantitative Trait Loci: Methods and Protocols. Edited by: N. J. Camp and A. Cox Humana Press, Inc., Totowa, ... foreach of the mother and father, and a code for sex (typically m and f or 1 and 2). In addition, trait and marker information are included. It is the combinationof family ID and individual ID that...
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Glycoprotein Methods and Protocols - P1
... Methods in Molecular BiologyTMHUMANA PRESSGlycoprotein Methods and Protocols Edited byAnthony P. CorfieldVOLUME 125The Mucins Methods in Molecular BiologyTMHUMANA ... MucinsGlycoprotein Methods and Protocols Isolation of Large Gel-Forming Mucins 11Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu-cins were mixed with DNA and subjected ... UV monitor and collect fractions using a fraction col-lector and subject to the appropriate carbohydrate and antibody analyses.3.6. Analysis of Mucins Methods for the detection and analysis...
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Glycoprotein Methods and Protocols - P2
... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucinstaining.1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa,NJ.50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties, Functions, and Applications ... such as Culling’s Handbook ofHistopathological and Histochemical Techniques (including museum techniques) (36) orKiernan’s Histological and Histochemical Methods: Theory and Practice (45) providecomprehensive...
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Glycoprotein Methods and Protocols - P3
... in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ.3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: The Mucins ... Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi-cation of mucins and their glycoforms, in Glycoprotein Methods and Protocols: TheMucins (Corfield, T., ... 1 and 2 (2,3) of this volume for the preparation ofsecreted and membrane-associated mucins, respectively, and to Chapter 7 (4) for adiscussion of methods for mucin separation.54 McGuckin and...
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Glycoprotein Methods and Protocols - P4
... GI Adherent Mucous Gel Barrier 5757From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press Inc., ... Allen and Pearsonrier and the major secretion of mucin of the gut, the adherent mucous gel is frequentlyignored because of the practical difficulties in observing and quantitating it. Quantitative ... poly-L-lysine for 5 min and dried at room temperature.3. Methods 3.1. Observation and Measurementof Mucous Thickness on Unfixed Mucosal Sections (see Notes 1–4)This is a rapid and simple method...
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Glycoprotein Methods and Protocols - P5
... Biosynthesis and Secretion of Mucin 6565From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield ... NJ6Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic LabelingJan Dekker, B. Jan-Willem Van Klinken,Hans A. Büller, and Alexandra W. C. Einerhand1. IntroductionMost epithelial ... PhosphorImager.4. The identification of mucin precursors and mature mucins using polypeptide-specificantisera and SDS-PAGE is elaborated in Chapters 20 and 21, and in several references(1,4–11). The precursor...
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Glycoprotein Methods and Protocols - P6
... small gel, use 1.6 g ofagarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL ofbuffer.2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... few separation techniques available to use with these77From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press Inc., ... Separation and Identification of Mucin 777Separation and Identificationof Mucins and Their GlycoformsDavid J. Thornton, Nagma Khan, and John K. Sheehan1. IntroductionThis...
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Glycoprotein Methods and Protocols - P7
... Heterogeneity and Size Distribution of Gel-Forming Mucins 8787From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. ... chapter describes only methods for assessing the heterogeneity of mucinpopulations with regard to mass and size utilizing rate zonal centrifugation and electronmicroscopy. Other methods for the absolute ... pump.4. Swing-out rotors and tubes (see Note 1).5. Gradient maker (see Note 2).896 Sheehan and Thornton12. Koller, T., Harford, A. G., Lee, Y. K., and Beer, M. (1969) New methods for the prepara-tion...
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Glycoprotein Methods and Protocols - P8
... 113113From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press Inc., Totowa, NJ10Amino Acid Analysis of MucinsJun X. Yan and ... caramelized and further charcoaled, and the acid hydrolysis results in ablack residue. This residue appears to precipitate protein/amino acids, interferes with114 Yan and Packerchromatography, and ... C18reversed-phase column and detected by fluorescence. Our routine analyses have shownthat the method described here is reliable and useful for both quantitative and qualitative mucin glycoprotein...
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Glycoprotein Methods and Protocols - P9
... Sequencing Glycopeptides 121121From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press Inc., ... 3 and 8) and the length of sequenceobtained is dependent on the number and distribution of proline residues. Proline cleaveswith reduced efficiency in Edman sequencing (Fig. 1, vessel 1), and ... Kirk, M., Barnes, S., Lowary, T. L., and Hart, G.W. (1996) Selective detection and site-analysis of O-GlcNAc-modified glycopeptides byβ-elimination and tandem electrospray mass spectrometry....
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