proteolytic enzymes, aspartic and metallo peptidases

... and Molecular Biology. In Volume 244 on peptidases of serine and cysteine type and in this volume on aspartic, metallo, and other peptidases, the chapters on spe- cific methods, enzymes, and ... DMSO, and at 25 ° for factor D, C2a~ and Bb, and 0.1 M HEPES, 10 mM CaC12, buffer, pH 7.5, 9% DMSO, and 30 ° for Clr and Cls. The units for kcat, Kin, and kcat/Km a...

Ngày tải lên: 11/04/2014, 10:13

... Union of Biochemistry and Molecular Biology. In Volumes 244 and its companion Volume 248 on aspartic and metallo peptidases, the chapters on specific methods, enzymes, and inhibitors are organized ... (Table I), bacteria, and eukaryotes, and they include exopeptidases, endo- peptidases, oligopeptidases, and omega peptidases. By the criteria we use to distinguish fam...

Ngày tải lên: 11/04/2014, 10:13

... The amino acid sequence enclosed in dark boxes and in open boxes are common sequences among ALP I and the neutral subtilisins, and among ALP I and the alkaline subtilisins, respectively. s, Unique amino ... Two mutant enzymes, D266N/Y269A- and D266N/Y269A/A271T/ Q272R-ALP I, were most stable retaining 60% of the original activity after 1 h, and 30% after 6 h. 2 The D266N- ALP I...

Ngày tải lên: 21/02/2014, 03:20

... atherosclerosis and acute lung injury. Both ACE and ACE2 have catalytic activity that is chloride sensitive and is caused by the presence of the CL1 and CL2 chloride-binding sites in ACE and the CL1 site ... system regulates blood pressure, electrolyte balance and fluid volume homeostasis [1]. ACE and ACE2 both belong to the M2 family (clan MA) of metalloproteases, and have the...

Ngày tải lên: 16/03/2014, 04:20

... of papain-like enzymes, the propeptide was incubated for 30 min with papain or human cathepsins B, L, S, K, V and F (in the subnanomolar range), and the residual activity of the peptidases was ... other enzymes, particularly surveying the residues and structural features at the PCZ–cruzipain contact interface that are conserved in brucipain and cathepsin F but not in cathepsins...

Ngày tải lên: 16/03/2014, 11:20

... incidents involving anesthesia. To under- stand the role of citrulline-NO cycle enzymes, GS and the oxidative status in anoxia and reperfusion, NOS, AS, AL, GS and arginase activities along with the ... acid transporter-2, and NOS in activated murine microglial cells (19) and co-induction of inducible NOS and arginine recycling enzymes in cytokine-stimulated PC12 cells and...

Ngày tải lên: 26/10/2012, 09:07

... 13% and 21% increase in the xylanase and cellulase production, respectively. The optimum temperatures of the crude cellulase and xylanase were 60 0 C and 70 0 C with half-lives of 15 h and ... degradation and conversion for sustainable production of bioenergy and high- value products; enzyme production and catalysis, bioremediation and biorefineries. Lew is the author of 7...

Ngày tải lên: 05/09/2013, 16:11

... overweight and obese _______ ∗ Corresponding author. Tel.: 84-4-38582179. E-mail: liendn@vnu.edu.vn persons and over 200 million persons suffered from diabetes mellitus (DM), and this number ... hypoglycemic and hypolipidemic activity have been described in D.N. Lien et al. / VNU Journal of Science, Natural Sciences and Technology 27 (2011) 118-124 119 the traditional re...

Ngày tải lên: 12/02/2014, 17:20

... 2-mercaptoethanol and dithiothreitol. For example, 1mm dithiothreitol caused 72% and 85% inhibition of BHLmL2 and BHAb, respectively, while 1 mm 2-mer- captoethanol caused 85% and 97% inhibition of BHLmL2 and ... oilseeds rapeseed and canola (Bras- sica napus and Brassica rapa) and vegetables such ase cabbage (Brassica oleraceae var. capitata), cauliflower (B. oleraceae var. bo...

Ngày tải lên: 18/02/2014, 14:20

... significant effects either. The dissociation rate constants for DHNP and DHMP were estimated from those for NP and MP and the difference between HP and HPO and finalized by iterative fittings as described in the ... NP, and MP were purchased from Schircks Laboratories (Jona, Switzerland). Restriction enzymes and T4 ligase were purchased from New England Biolabs (Ipswich, MA, USA)....

Ngày tải lên: 19/02/2014, 00:20